| Chemoresistance of CD133（＋） tumor stem cells from human brain glioma |
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| 作者姓名： | Bi CL Fang JS Chen FH Wang Y J Wu J |
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| 作者单位： | Department of Neurosurgery, Xiansya Hospital, Central South University, Changsha 410008, China. |
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| 摘 要： | OBJECTIVE： To explore the multidrug resistance （MDR） mechanism of ABC superfamily transporters in the tumor stem eells（TSC） from human brain ghoma tissues. METHODS： Samples of glioma were obtained from 30 patients undergoing microsurgical tumor resection. The CD133（＋） cells and CD133（-） cells from these tumor specimens were isolated by magnetic activated cell sorting（MACS）. These cells were cultured, proliferated and passaged. The protein and activity expression of multidrug-resistance protein 1 （MDR1） and multidrug-resistance associated protein 1（MRP1） were analyzed between CD133（＋） and CD133（-） cells by immunocytochemistty and RT-PCR respectively.[第一段]
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| 关 键 词： | 肿瘤干细胞 神经胶质瘤 抗药性 CD133（＋） |
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