顶空气相色谱法测定鸡骨草总黄酮碳苷提取物中大孔吸附树脂残留物

目的：建立同时测定鸡骨草总黄酮碳苷提取物中8种大孔吸附树脂残留物的顶空气相色谱分析方法。方法：采用Supelco-wax毛细管柱（30m×0．25ram×0．25μm），体积分数为50％的N，N-二甲基甲酰胺（DMF）为溶解介质，高纯氮作为栽气，FID检测器；进样口温度210℃，检测器温度230℃，柱温先在70℃维持8min，再以10℃／min的速度升至220℃，维持7min。结果：在以上色谱条件下，各组分在所考察的浓度范围内均具有良好的线性，r为0．9965～0．9999，RSD为1．839％～9．155％，苯、正己烷、甲苯、间二甲苯、邻二甲苯、对二甲苯、二乙烯苯及苯乙烯的最低检出浓度分别为0．05、0．25、0．1、0．1、0．1、0．1、0．2、0．1μg·mL-1。结论：该法快速、灵敏、准确，适用于鸡骨草总黄酮碳苷提取物中大孔吸附树脂残留物的测定。

Determination of Macroporous Resin Residues in Total Flavonoid C-Glycosides Extracts of Abrus Mollis by Headspace GC

Objective：To establish a headspace GC method for simultaneous determination of the eight kinds of macroporous resin residues in total flavonoid C-Glycosides extracts of Abrus mollis. Methods： The determination was performed on Supelco-Wax N, N-dimethylformamide（DMF） and the detector was FID; temperature column（30m × 0.25mm × 0.25μm）. The solvent medium was 50 % , The injector was, the detector temperature was 230℃, and carrier gas was high-purity nitrogen. The column temperature was changed according to the program：initial temperature was kept at 70℃ for 8min, then raised to 220℃ at the rate of 10℃/rain, and maintained for 7min. Results. The results showed good linearity （r=0. 996 5-0. 999 9）,and relative standard derivations （RSD） of precision were in the range of 1. 839 %-9. 155 %. The minimum detection concentrations of benzene, n-hexane, Methyl benzene, m- xylene, o-xylene, p-xylene,divinylbenzene and styrene were 0.05,0.25, 0.1, 0.1, 0.1, 0.1, 0.2and 0. lμg . mL-1 ,respectively. Conclusion ： The method is simple, sensitive and accurate. It is suitable for determination of macroporous resin residues in total fla vonoid C-Glycosides extracts of Abrus mollis.