卵巢颗粒膜细胞一氧化氮的分泌及激素调节作用

目的探讨排卵前人卵巢颗粒膜细胞一氧化氮（NO）的分泌与白细胞介素1β（IL-1β）的关系,及其调节雌、孕激素的作用。方法收集体外受精-胚胎移植时穿刺卵巢抽吸的卵泡液,取出卵细胞后,分离出颗粒膜细胞进行培养,分别进行向其内添加IL-1β及NO供体的实验,测定各自培养液中的NO、雌二醇(E2)、孕酮(P)量及芳香化酶的活性。结果添加IL-1β组颗粒膜细胞的NO分泌量为（537±23）μmol/L,不添加IL-1β组为（411±15）μmol/L（P<0.01）;添加NO供体S-硝基乙酰基青霉胺（SNAP）组颗粒膜细胞的E2分泌量为（1234±136）μg/L,不添加NO供体SNAP组为（1792±103）μg/L（P<0.01）;添加NO供体SNAP组颗粒膜细胞的P分泌量为（988±103）μg/L,不添加NO供体SNAP组为（2486±123）μg/L（P<0.001）;添加NO供体SNAP组颗粒膜细胞的芳香化酶活性为（0.422±0.052）Ci/ml,不添加NO供体SNAP组为（0.833±0.012）Ci/ml（P<0.01）。结论在排卵前,卵巢颗粒膜细胞可分泌NO并可能受IL-1β的调节,NO与IL-1β共同参与类固醇激素合成的调节。

Secretion and regulation of nitric oxide in ovarian granular cell

OBJECTIVE: To investigate the relationship between nitric oxide (NO) secreted by ovarian granular cell and interleukin-1 beta (IL-1 beta), and their functions in estrogen synthesis. METHODS: The human ovarian granular cells recovered from follicular fluid were cultured with IL-1 beta or NO donor. RESULTS: The quantity of NO cultured with or without IL-1 beta addition were (537 +/- 23) mumol/L, (411 +/- 15) mumol/L respectively. The former was significantly higher than the latter (P < 0.01). The estradiol, progesterone synthesis and aromatizing enzyme activity in the media with the addition of NO donor were (1,234 +/- 136) micrograms/L, (988 +/- 103) micrograms/L, (0.422 +/- 0.052) Ci/ml, while as they were (1,792 +/- 103) micrograms/L, (2,486 +/- 123) micrograms/L, (0.833 +/- 0.012) Ci/ml, in the media without the addition of NO donor. The estradiol progesterone synthesis and aromatizing enzyme activity in the media with NO donor decreased significantly as compared with the group without NO donor (P < 0.01, P < 0.001, P < 0.01). CONCLUSIONS: Ovarian granulosa cells may secrete NO, and the secretion may be regulated by IL-1 beta. They may be involved in the ovulation at the preovulatory stage and regulation of ovary steroids synthesis.