Antitumor effects and chemical compositions of Eupolyphaga sinensis Walker ethanol extract |
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Authors: | Ge Gang-Feng Yu Chen-Huan Yu Bing Shen Zhen-Hua Zhang Dong-Liang Wu Qiao-Feng |
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Affiliation: | College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. |
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Abstract: | Ethnopharmacological relevanceEupolyphaga sinensis Walker popularly known as “preferred drug to regulate blood flow” are traditionally used in folk medicine in the treatment of ecchymoma, posttraumatic wound, hepatic fibrosis and tumor.Aim of the studyTo characterize chemical compositions and to evaluate the antitumor and immunomodulatory of Eupolyphaga sinensis Walker ethanol extract (ESEE) in hepatocarcinoma H22 bearing mice.Materials and methodsESEE was obtained by ethanol reflux extraction and analyzed by gas chromatography–mass spectrometry (GC–MS) after methylation. ICR mice were treated with ESEE for 14 consecutive days at doses of 31 mg/kg (low-dose), 62 mg/kg (mid-dose) and 124 mg/kg (high-dose) after H22 tumor cells were implanted. At the end of the experiments, the tumor weight of each mouse was measured. Levels of serum TNF-α and IFN-γ was assayed by ELISA. Protein expressions of Bax, Bcl-2 and caspases-3 were detected by immunohistochemistry.ResultsChemical analysis revealed the presence of 6 components that account for 97.55% of fatty acids, indicating the occurrence of saturated and polyunsaturated fatty acids. Oral administration of ESEE could inhibit tumor growth, promote Th1 type cytokine productions (TNF-α and IFN-γ) and induce apoptosis of hepatocarcinoma via increase of Bax/Bcl-2 ratio and activation of caspases-3. Oral administration of ESEE in a dosage of 6.2 g/kg did not lead to toxic effects in mice.ConclusionsESEE was effective in inhibiting tumor growth in vivo and could also serve as immunoadjuvant for tumor therapy. |
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Keywords: | ESEE, Eupolyphaga sinensis Walker ethanol extract Fu, fluorouracil NC, normal control MC, model control, H22 tumor bearing mice MC + Fu, H22 tumor bearing mice treated with fluorouracil MC + ESEE, H22 tumor bearing mice treated with ESEE Bcl-2, B-cell lymphoma 2 Bax, Bcl-2 antagonist X |
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