| 冬凌草甲素对MCF-7细胞的生长抑制作用及诱导凋亡作用的实验研究 |
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| 引用本文： | 朱仁书,李希科. 冬凌草甲素对MCF-7细胞的生长抑制作用及诱导凋亡作用的实验研究[J]. 医药论坛杂志, 2009, 0(16): 3-5 |
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| 作者姓名： | 朱仁书 李希科 |
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| 作者单位： | 鹤壁职业技术学院护理系,河南,鹤壁市,458030;鹤壁职业技术学院护理系,河南,鹤壁市,458030 |
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| 摘 要： | 目的探讨冬凌草甲素对人乳腺癌细胞MCF-7细胞的生长抑制作用及诱导凋亡作用。方法以不同浓度的冬凌草甲素作用于体外培养的MCF-7细胞,MTT法检测细胞生长抑制率,应用Hoechst 33258荧光染色法以及流式细胞仪观察细胞凋亡情况。结果冬凌草甲素可显著抑制MCF-7细胞的生长及诱导细胞发生凋亡,并呈现出一定的剂量-效应与时间-效应关系。Hoechst 33258荧光染色观察到典型的核浓缩、核碎裂等细胞凋亡的形态学变化。结论冬凌草甲素能显著抑制MCF-7细胞的生长,诱导细胞发生凋亡是其重要作用方式。
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| 关 键 词： | 冬凌草甲素 MCF-7细胞 生长抑制 细胞凋亡 |
Growth Inhibitory Effect and Inducing Apoptosis Effect of Oridonin on Human Breast Cancer MCF-7 Cells |
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| ZHU Renshu,LI Xike. Growth Inhibitory Effect and Inducing Apoptosis Effect of Oridonin on Human Breast Cancer MCF-7 Cells[J]. Journal of Medical Forum, 2009, 0(16): 3-5 |
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| Authors: | ZHU Renshu LI Xike |
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| Affiliation: | ZHU Renshu,LI Xike Department of Nursing,Hebi Vocational , Technical College,Hebi 458030,China |
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| Abstract: | Objective To investigate the growth inhibitory effect and inducing apoptosis effect of oridonin on MCF-7 cells.Methods Different concentrations of oridonin were added in culture medium.The growth inhibitory rate of the MCF-7 cells was measured by MTT assay,cell apoptotic rate was detected by flow cytometry(FCM),morphology of apoptosis was observed by Hoechst 33258 fluorescence staining.Results Oridonin could inhibit the growth of MCF-7 cells and induce cell apoptosis significantly.The suppression was both i... |
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| Keywords: | Oridonin MCF-7 cells Growth inhibition Apoptosis |
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