兴奋性氨基酸拮抗剂对离体皮质神经细胞缺氧损伤的保护作用

近年来，许多研究的结果显示，兴奋性氨基酸谷氨酸，天冬氨酸等通过其受体介导了缺氧，缺血性脑损伤，同时兴奋性氨基酸受体拮抗剂对缺氧，缺血性脑损伤有一定的保护作用，本文研究了离体培养的小鼠皮层神经细胞的缺氧模型，并观察缺氧和兴奋性氨基酸受体激动剂Ｎ－甲基Ｄ－天冬氨酸（ＮＭＤＡ）对神经细胞的损伤作用，同时应用兴奋性氨基酸ＮＭＤＡ受体拮抗剂ＣＰＰ，Ｋｅｔ，及非ＮＭＤＡ受体拮抗剂ＮＢＱＸ观察其对缺氧及ＮＭＤＡ

Excitatory amino acid antagonists prevent cortical neurons injury in vitro

in recent years,the results of many experiments show that excitatory amino acids such as glutamate and aspartate mediate hypoxic-ischemic brain damage by its receptors,and excitatory amino acid antagonists can prevent hypoxic-ischemic brain damage.This experiment studied the hypoxia model in murine cortical cell culture,the neuronal injury caused by hypoxia and excitatory amino acid agonist NMDA,and the preventive effect of NMDA antagonists CPP,Ket and non-NMDA antagonist NBQX.The result indicated that (1)the NMDA receptor of excitatory amino acids mediated hypoxia neuronal damage;(2)both NMDA antagonists CPP,Ket and non-NMDA antagonist NBQX prevented neurons from hypoxia injury. Our study suggests that excitatory amino acid antagonists will be the new clinical therapeutic passway of brain ischemia.