| 石见穿多糖的含量测定及组分分析 |
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| 引用本文： | 徐伟,郑海音,郑晓燕,汤须崇.石见穿多糖的含量测定及组分分析[J].福建中医学院学报,2008,18(1):20-22. |
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| 作者姓名： | 徐伟 郑海音 郑晓燕 汤须崇 |
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| 作者单位： | 1. 福建中医学院药学系,福建,福州,350108
2. 福建中医学院中西医结合系,福建,福州,350108 |
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| 摘 要： | 目的测定石见穿多糖的含量及组分分析。方法用蒽酮-硫酸法测定石见穿含量；用薄层层析法分析石见穿多糖的组分。结果在2．18-10.90μg／ml内浓度与吸收度呈良好线性，回归方程A=0．0367C＋0.0412，r=0．9998，平均回收率为97.99％，RSD为1．28％；石见穿多糖的含量为3．15％。结论蒽酮-硫酸法测定石见穿，方法简便，结果准确，可作为石见穿质量的评价依据。
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| 关 键 词： | 石见穿 多糖测定 蒽酮-硫酸法 组分分析 |
| 文章编号： | 1004-5627（2008）01-0020-03 |
| 收稿时间： | 2007-09-05 |
| 修稿时间： | 2007年9月5日 |
Determination of Polysaccharides in Salvia Chinensis by Anthranone-sulfuric Acid and Constituents Analysis |
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| XU Wei, ZHENG Hai-yin, ZHENG Xiao-yan, et al.Determination of Polysaccharides in Salvia Chinensis by Anthranone-sulfuric Acid and Constituents Analysis[J].Journal of Fujian College of Traditional Chinese Medicine,2008,18(1):20-22. |
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| Authors: | XU Wei ZHENG Hai-yin ZHENG Xiao-yan |
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| Institution: | XU Wei, ZHENG Hai-yin, ZHENG Xiao-yan, et al (1. Department of pharmacy, FUTCM, Fuzhou, Fujian 350108, China; 2. Department of Integrative Medicine, FUTCM, Fuzhou, Fujian 350108, China) |
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| Abstract: | Objective To determine the polysaccharides in Salvia chinensis Benth. Methods The polysaccharides of Salvia chinensis were determined by anthranone-sulfuric acid method and their components were analized by TLC. Results There was an excellent linear shape between the concentration and absorptance in the range of 2.18 to 10.90 μg/ml, the regression equation was A=0.0367C＋0.0412, r= 0.9998, and the average recovery was 97.99% with RSD of 1.28% （n=9）, the content of the polysaccharides was 3.15%. Conclusion This method is simple and accurate. |
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| Keywords: | Salvia chinensis determination of polysaccharides anthranone-sulfuric acid method component analysis |
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