| ELISA法检测单纯疱疹病毒Ⅱ型特异性抗体的临床应用研究 |
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| 引用本文： | 杨慧兰,杨军,刘荣卿. ELISA法检测单纯疱疹病毒Ⅱ型特异性抗体的临床应用研究[J]. 第三军医大学学报, 2000, 22(4): 383-384 |
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| 作者姓名： | 杨慧兰 杨军 刘荣卿 |
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| 作者单位： | 广州军区总医院皮肤科!广州510010(杨慧兰，杨军)，第三军医大学附属西南医院皮肤科!重庆400038(刘荣卿)，广州军区总医院医学实验科!广州510010(杨太成，王捷，冼江，李建军) |
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| 基金项目： | 广东省科委自然基金!资助项目(960671) |
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| 摘 要： | 目的 探讨单纯疱疹病毒Ⅱ型（ＨＳＶ－２）感染的早期特异检测手段。方法 对５２例性病门诊的生殖器疱疹患者改良的ＥＬＩＳＡ方法进行了ＨＳＶ－ｇＤ２ＩｇＧ检测并同细胞分离方法进行对比观察。结果 病毒分离的阳性率为６９．２％，ＥＬＩＳＡ法检测抗体的阳性率６５％，两种方法检测结果差异无显著性。结论：ＥＬＩＳＡ法检测血清疱疹病毒ＩｇＧ抗体具有简便，快速，特异的优点，适合于临床大规模检测之有
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| 关 键 词： | 单纯疱疹病毒 Ⅱ型 糖蛋白D ELISA |
Detection of herpes simplex virus glycoprotein D_2 IgG |
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| YANG Hui-lan,YANG Jun,LIU Rong-qing,YANG Tai-cheng,WANG Jie,XIAN Jiang,LI Jian-jun. Detection of herpes simplex virus glycoprotein D_2 IgG[J]. Acta Academiae Medicinae Militaris Tertiae, 2000, 22(4): 383-384 |
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| Authors: | YANG Hui-lan YANG Jun LIU Rong-qing YANG Tai-cheng WANG Jie XIAN Jiang LI Jian-jun |
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| Abstract: | Objective To explore a specific method for the early diagnosis of genital herpes. Methods 52 specimens were collected from the outpatient department of venereal disease and examined with ELISA for the detection of Herpes simplex virus glycoprotein D2 IgG and cell isolation culture was done for comparison. Results The positive rate of the detection of the specific antibody against Herpes simplex virus was 69.2% and that of the isolation of the virus was 65%. Conclusion ELISA is the technique of choice to be used in outpatient clinic to detect Herpes simplex virus glycoprotein D2 IgG. |
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| Keywords: | s: Herpes simplex virus glycoprotein D2 IgG ELISA |
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