前列腺素E1对犬肺过度膨胀所致损伤保护作用的实验研究

目的 探讨前列腺素E1对肺过度膨胀所致损伤的保护作用及作用机制.方法 杂种犬16只,随机分为对照组和实验组,建立肺过度膨胀动物模型.对照组保持肺过度膨胀6 h;实验组保持肺过度膨胀6 h,并以30 ng/(kg·min)的剂量给予前列腺素E1,6 h后取标本进行肺组织湿/干比、支气管肺泡灌洗液中蛋白含量的检测,并进行肺组织光镜和电镜的观察,观察肺损伤的变化情况.结果 对照组肺组织湿/干比、支气管肺泡灌洗液中蛋白含量(BALF)明显高于实验组(P＜0.05);肺组织学检查实验组肺损伤程度明显轻于对照组.结论 前列腺素E1对犬肺过度膨胀所致损伤有明显的保护作用,其作用机制与抑制中性粒细胞活性,降低中性粒细胞、血小板聚集等有关.

Experimental study on protective effect of prostaglandin E1 on lung hyperinflation injury in dogs

Objective To explore the protective effect of prostaglandin E_1(PGE_1) on lung and its possible mechanism during lung hyperinflation injury.Methods Sixteen mongrels were randomly divided into control group(C)and PGE-n=1 group(P)and lung hyperinflation canine model was established.Lung hyperinflation was kept in control group for 6 hours,and on the basis of the former the PGE_1 group was given 30 ng/kg/min PGE_1.The changes of lung tissue wet weight/dry weight ratio(W/D)and total protein content of bronchoalveolar lavage fluid(BALF) were measured after 6 hours; the histological appearances of the lung tissue and the pulmonary vascular were observed under histology and electron microscopy.Results W/D and total protein content of BALF were significantly higher in control group than in PGE_1 group(P<0.05); histological examination of lung tissue demonstrated that the degree of lung injury was worsen in PGE_1 group than in control group.Conclusion PGE_1 can significantly attenuate the lung hyperinflation injury,which protective mechanism may be that PGE_1 can inhibit aggregation of neurotrophilic granulocyte and platelet in the lung.