乙型肝炎病毒前S2基因克隆及真核表达载体的构建

目的:克隆乙型肝炎病毒前S2基因,构建其真核表达质粒.方法:以乙型肝炎患者血清HBV DNA为模板,设计引物扩增全长前S2基因,再将目的基因插入到真核载体pcDNA3.1(+)中,经PCR、酶切和DNA测序确认.结果:从患者血清抽提的DNA中扩增得到约860bp大小的目的片段,经回收、纯化、酶切后转化大肠杆菌DH5α,...

Cloning of Hepatitis B Virus PreS2 Gene and Construction of Eukaryotic Expression Vector

Objective:To clone HBV preS2 gene and construct an eukaryotic expressive vector pcDNA-S2.Method:HBV preS2 gene was amplified by PCR from serum of a chronic HBV carrier,and cloned into the eukaryotic vector pcDNA3.1(+),then authenticated by PCR,digestive enzymes and sequencing.Results:A 860bp fragment of the preS2 gene was successfully amplified by PCR from the serum,and inserted into the eukaryotic vector pcDNA3.1(+). The positive clone was identified by PCR,enzyme digestion and DNA sequencing.Conclusion:Eu...