| 黄芪-脉络宁胶原促进体外胸主动脉内皮细胞血管新生的实验研究 |
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| 引用本文： | 薛涛,陈建东,姚昶. 黄芪-脉络宁胶原促进体外胸主动脉内皮细胞血管新生的实验研究[J]. 微循环学杂志, 2011, 21(1): 8-11,80,83,75 |
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| 作者姓名： | 薛涛 陈建东 姚昶 |
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| 作者单位： | 1. 东南大学附属中大医院胸心外科,南京,210009
2. 江苏省中医院 |
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| 基金项目： | 江苏省自然科学基金(BK2007175); 江苏省“六大人才高峰”(06-B-071) |
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| 摘 要： | 目的:探讨黄芪-脉络宁胶原对血管新生的细胞学影响及其可能机制,为其应用于临床提供理论依据。方法:应用增殖、迁移、管腔形成实验观察黄芪-脉络宁胶原对大鼠胸主动脉内皮细胞在血管新生各阶段的作用,用Western Bloting实验检测大鼠胸主动脉血管内皮细胞生长因子(VEGF)的表达率。结果:黄芪-脉络宁胶原能明显促进大鼠胸主动脉内皮细胞的增殖率(与对照组比较,增加了90.22%,P<0.01)、迁移率(与对照组比较增加了211.43%,P<0.01)、管腔形成数(是对照组的2.46倍,P<0.01),同时刺激VEGF蛋白表达比对照组增加63.8%(P<0.01)。结论:黄芪-脉络宁胶原能明显促进内皮细胞增殖、迁移、管腔形成,具有显著促体外血管新生作用,其机制可能与增加VEGF蛋白的表达有关。
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| 关 键 词： | 胶原蛋白 血管新生 血管内皮细胞生长因子 黄芪-脉络宁 |
Enhancing Angiogenesis of Thoracic Aorta Endothelial Cells by Loading Huangqi-Maluoning Injection Into Modified Collagen Matrices |
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| Xue Tao,Chen Jiandong,Yao Chang. Enhancing Angiogenesis of Thoracic Aorta Endothelial Cells by Loading Huangqi-Maluoning Injection Into Modified Collagen Matrices[J]. Chinese Journal of Microcirculation, 2011, 21(1): 8-11,80,83,75 |
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| Authors: | Xue Tao Chen Jiandong Yao Chang |
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| Affiliation: | Xue Tao,Chen Jiandong,Chang Yao/Department of Cardiothoracic Surgery,Zhongda Hospital of Southeast University,Nanjing 210009 |
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| Abstract: | Objective:To explore the angiogenic effects and possible mechanisms of modified collagen matrices loading with Huangqi-Maluoning injection with cell experiments.Method:2 groups of collagen matrices loading with or without Huangqi-Mailuoning injection were set in the experiments,each of which was cultured with thoracic aorta endothelial cells for 24 hours. Afterwards,the effects of cell proliferation,migration and tube formation were detected followed by protein expression of VEGF with western blot assay.Res... |
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| Keywords: | Collagen Angiogenesis VEGF Huangqi-Maluoning |
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