Autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells |
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Authors: | Li Wei-Ren Chen Liang Chang Zhi-Jie Xin Hua Liu Tao Zhang Yan-Quan Li Guang-Yong Zhou Feng Gong Yan-Qing Gao Zhe-Zhu Xin Zhong-Cheng |
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Institution: | Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China. |
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Abstract: | Late-onset hypogonadism (LOH) is closely related to secondary androgen deficiency in aged males, but the mechanism remains unclear. In this study, we found that reduced testosterone production in aged rat Leydig cells is associated with decreased autophagic activity. Primary rat Leydig cells and the TM3 mouse Leydig cell line were used to study the effect of autophagic deficiency on Leydig cell testosterone production. In Leydig cells from young and aged rats, treatment with wortmannin, an autophagy inhibitor, inhibited luteinising hormone (LH)-stimulated steroidogenic acute regulatory (StAR) protein expression and decreased testosterone production. In contrast, treatment with rapamycin, an autophagy activator, enhanced LH-stimulated steroidogenesis in Leydig cells from aged, but not young, rats. Intracellular reactive oxygen species (ROS) levels were increased in both young and aged Leydig cells treated with wortmannin but decreased only in aged Leydig cells treated with rapamycin. Furthermore, an increased level of ROS, induced by H(2)O(2), resulted in LH-stimulated steroidogenic inhibition. Finally, knockdown of Beclin 1 decreased LH-stimulated StAR expression and testosterone production in TM3 mouse Leydig cells, which were associated with increased intracellular ROS level. These results suggested that autophagic deficiency is related to steroidogenic decline in aged rat Leydig cells, which might be influenced by intracellular ROS levels. |
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Keywords: | ageing autophagy late onset hypogonadism Leydig cell reactive oxygen species testosterone |
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