实验性脑出血后高血糖致能量代谢障碍加重脑损伤的可能机制

目的:观察大鼠脑出血(ICH)后高血糖对血肿周围脑组织含水量和三磷酸腺苷(ATP)的影响,探讨ICH后高血糖对脑损伤的可能机制。方法:采用自体血注入法建立脑出血模型,将100只SD大鼠随机分成假手术组(SO)、单纯脑出血组(PCH)、ICH后高血糖组,ICH后高血糖组又分为2g/kg(2-GS)和4g/kg(4-GS)注射葡萄糖组。观察各组大鼠术前、术后1h、6h血糖变化;测定各组大鼠术后1、3、7、10、14d血肿周围脑组织含水量和ATP含量变化。结果:与SO组比较,脑出血3组血肿周围脑组织含水量升高,ATP含量降低(P<0.05);与PCH组比较,2-GS组和4-GS组大鼠血肿周围脑组织含水量明显升高(P<0.05),ATP含量明显降低(P<0.05)。结论:提示脑出血后高血糖导致能量代谢障碍,可能通过加重脑水肿而加重脑损伤。

HIGH BLOOD SUGAR EXAGGERATE BRAIN DAMAGE IN EXPERIMENTAL BRAIN HEMORRHAGE

Objective:To observe the effect of high blood glucose on water and adenosine triphosphate(ATP) content changes around hematoma in rats with intracerebral hemorrhage(ICH),and to explore its possible mechanism of brain injury.Methods:ICH model was established through autologous blood injection.100 SD rats were randomly divided into sham operation group(SO),cerebral hemorrhage group(PCH),cerebral hemorrhage plus hyperglycemia 2g/kg(2-GS) group,4g/kg(4-GS) group.Before and 1h,6h after operation blood glucose changes were observed;1,3,7,10,14d afted operation perihematoma water content and ATP levels were measured.Results: Compared with SO group,the other 3 groups with brain hemorrhage had significantly higher water content and significantly lower ATP content(P<0.05);compared with PCH group,2-IG and 4-IG group had significantly higher water content,and lower ATP content(P<0.05).ConclUSion:Hyperglycemia after cerebral hemorrhage can cause disorder of energy metabolism,and brain edema may aggravate brain damage.