| HPLC法测定川产道地药材雅连中盐酸小檗碱的含量 |
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| 引用本文： | 李春红,张金平,何兵,田吉.HPLC法测定川产道地药材雅连中盐酸小檗碱的含量[J].泸州医学院学报,2012,35(2):145-147. |
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| 作者姓名： | 李春红 张金平 何兵 田吉 |
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| 作者单位： | 1. 泸州医学院药物与功能性食品研究中心,四川泸州,646000
2. 泸州医学院基础医学院病原生物学教研室,四川泸州,646000 |
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| 摘 要： | 目的:建立川产道地药材雅连中盐酸小檗碱的含量测定方法。方法:采用HPLC法,色谱柱为依利特SinoChrom ODS-BP(250mm×4.6mm,5μm),流动相为乙腈-水(含0.1%乙酸和10mmol/L乙酸铵)梯度洗脱,检测波长345 nm,柱温30℃;流速1.0ml/min;进样量:10μl。结果:盐酸小檗碱在0.25～2.5μg范围内与峰面积线性关系良好(r=0.9999),平均回收率99.29%,RSD为1.38%(n=9)。结论:本方法灵敏、准确、简便快速,可用于川产道地药材雅连的质量控制。
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| 关 键 词： | 雅连 盐酸小檗碱 含量测定 高效液相色谱法 |
Determination of berberine hydrochloride in Coptis deltoidea C.Y.Cheng et Hsiao in Sichuan by HPLC |
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| Institution: | Li Chunhong,et al Drug and Functional Food Research Center of Luzhou Medical College |
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| Abstract: | Objective:To establish a method for the determination of berberine hydrochloride in Coptis deltoidea C.Y.Cheng et Hsiao in Sichuan.Methods:The separation was carried out on a SinoChrom ODS-BP(250mm×4.6mm,5μm),the mobile phase was acetonitrile-water(0.1%caproic acid and 10 mmol/L ammonium acetate) with gradient elution,and the detected wavelength was 345nm.The column temperature was 30℃.The flow rate was 1.0 ml/min,and the sample size was 10μl.Results:The calibration curve showed good linearity over the range of 0.25～2.5μg(r=0.9999).The average recovery was 99.29 % with RSD 1.38%(n=9).Conclusion:The method is sensitive,accurate,rapid,and it is suitable for the quality control of Coptis deltoidea C.Y.Cheng et Hsiao. |
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| Keywords: | Coptis deltoidea C Y Cheng et Hsiao Berberine hydrochloride Content determination HPLC |
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