4NQO饮水法诱发小鼠头颈部鳞状细胞癌模型的建立

目的快速建立头颈部鳞状细胞癌（简称鳞癌）动物模型。方法Balb/c小鼠65只,随机分为A、B、C、D 4组,分别有20、20、20、5只小鼠,A、B、C 3组为实验组,分别用0.002%、0.005%、0.01%的4-硝基喹啉-1-氧化物（4-nitroquinoline-1-oxide,4NQO）饮水喂养,D组为对照组饮用自来水。肉眼及组织学观察比较4组小鼠癌变过程。结果A、B、C各组分别于第16、11、9周出现舌背黏膜异常增生。到32周A组小鼠舌黏膜中重度异常增生53.8%,原位癌23.1%,浸润癌23.1%;B组小鼠舌黏膜重度异常增生8.3%,原位癌1 6.7%,浸润癌75%;C组小鼠舌黏膜原位癌8.3%,浸润癌91.7%;D组小鼠均无舌黏膜病变。随饮用4NQO剂量增加,小鼠舌背黏膜异常增生程度越重（P〈0.01）。结论4NQO饮水法诱发小鼠头颈部鳞癌,组织病理学特征与人头颈部鳞癌相似,随致癌剂作用时间延长和4NQO浓度的增加,小鼠舌黏膜异常增生越严重。0.01%的4NQO饮用23～32周是建立头颈部浸润鳞癌动物模型的适宜方法。

A mouse model of head and neck squamous cell carcinoma induced by 4-nitroquinoline-l-oxide

OBJECTIVE To rapidly establish a mouse model with head and neck squamous cell carcinoma.METHODS 65 Balb/c mice were randomized into 4 groups(A,B,C and D).Animals in group A,B and C(each contained 20 mice) were fed with 0.002%,0.005%and 0.01%4NQO solution respectively.Group D was the control group,which had 5 mice and the animals were fed with tap water. The tongue mucosal changes were observed through both naked eye and histopathological examination. RESULTS The dorsal tongue mucosal dysplasia of the mice i...