| 一氧化氮、诱生型一氧化氮合酶和血管内皮生长因子C在鼻咽癌中的作用 |
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| 引用本文： | 易翔,唐安洲,覃颖,谢莹,温文胜.一氧化氮、诱生型一氧化氮合酶和血管内皮生长因子C在鼻咽癌中的作用[J].中国耳鼻咽喉头颈外科,2009(12):673-677. |
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| 作者姓名： | 易翔 唐安洲 覃颖 谢莹 温文胜 |
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| 作者单位： | 广西医科大学第一附属医院耳鼻咽喉头颈外科;广西壮族自治区人民医院耳鼻咽喉科2区; |
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| 摘 要： | 目的检测鼻咽癌组织中诱生型一氧化氮合酶（inducible nitric oxide synthase,iNOS）及血管内皮生长因子C（vascular endothelial growth factor C,VEGF-C）表达的意义以及它们之间的关系,并检测一氧化氮（nitric oxide,NO）对鼻咽癌细胞株生长的影响及对VEGF-C表达的调节作用。方法用免疫组化法检测57例鼻咽癌标本以及20例非鼻咽癌鼻咽上皮组织标本中VEGF-C以及iNOS的表达,另以NO供体S-亚硝基-N-乙酰基-DL-青霉胺（S-nitroso-N-acetylpenicillamine,SNAP）对鼻咽癌细胞系TWO3进行处理,通过MTT法检测NO对TWO3生长的影响,并通过逆转录聚合酶链反应（RT-PCR）法检测NO对TWO3的VEGF-C表达调控作用。结果VEGF-C和iNOS在鼻咽癌组中表达阳性率显著高于对照组（P〈0.05）,鼻咽癌组织中VEGF-C表达在不同T、N以及临床分期组差异有统计学意义（P〈0.05）,但是不同年龄性别组表达差异无统计学意义（P〉0.05）。鼻咽癌组织中iNOS的表达在不同T、N级,临床分期,年龄以及性别组中表达差异无统计学意义（P〉0.05）;鼻咽癌组织中iNOS与VEGF-C表达显著相关（P〈0.05）;高浓度NO能抑制TWO3生长,且NO能调节TWO3的VEGF-C的转录表达。结论VEGF-C及iNOS可能对鼻咽癌的发展起促进作用,且在鼻咽癌中NO可能调节VEGF以及VEGF-C表达调节。
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| 关 键 词： | 鼻咽肿瘤 血管内皮生长因子C 一氧化氮合酶 一氧化氮 细胞系 |
Effect and relationship of inducible nitric oxide synthase,nitric oxide and vascular endothelial growth factor C in nasopharyngeal carcinoma |
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| YI Xiang,TANG Anzhou,QIN Ying,XIE Ying,WEN Wensheng.Effect and relationship of inducible nitric oxide synthase,nitric oxide and vascular endothelial growth factor C in nasopharyngeal carcinoma[J].Chinese Archives of Otolaryngology-Head and Neck Surgery,2009(12):673-677. |
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| Authors: | YI Xiang TANG Anzhou QIN Ying XIE Ying WEN Wensheng |
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| Institution: | YI Xiang1,TANG Anzhou1,QIN Ying2,XIE Ying1,WEN Wensheng1(1 Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi,530021,China,2 Department of Otolaryngology,People's Hospital of Guangxi Zhuang Autonomous Region,China) |
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| Abstract: | OBJECTIVE To study the expression of inducible nitric oxide synthase(iNOS)and vascular endothelial growth factor C(VEGF-C) in nasopharyngeal carcinoma(NPC).To study the effect of nitric oxide(NO)on NPC cell line TWO3 growth and modulation of VEGF-C expression in TWO3.METHODS The expression of iNOS and VEGF-C in 57 nasopharyngeal carcinoma(NPC) tissues was detected by immunohistochemistry matched with 20 non nasopharyngeal tumor tissues as control.The effect of NO on NPC cell line TWO3 growth and modulation ... |
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| Keywords: | Nasopharyngeal Neoplasms Vascular Endothelial Growth Factor C Nitric Oxide Synthase Nitric Oxide Cell Line |
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