| 三种病毒载体经蜗窗膜导入大鼠耳蜗的靶向分布比较 |
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| 引用本文： | 雷雳,韩德民. 三种病毒载体经蜗窗膜导入大鼠耳蜗的靶向分布比较[J]. 中国耳鼻咽喉头颈外科, 2009, 16(11): 608-610 |
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| 作者姓名： | 雷雳 韩德民 |
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| 作者单位： | 北京同仁医院耳鼻喉科 |
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| 基金项目： | 国家自然科学基金资助项目(30471869,30772406) |
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| 摘 要： | 目的比较3种病毒载体经蜗窗膜注入大鼠耳蜗鼓阶介导报告基因在内耳的转染分布,甄选螺旋神经节细胞及其神经纤维基因靶向转移载体。方法分别将带有绿色荧光蛋白基因的慢病毒、5型-腺病毒、2型-腺相关病毒载体经大鼠耳蜗蜗窗膜缓慢注入鼓阶外淋巴,荧光显微镜观察病毒载体导入后3、7、14天绿色荧光蛋白基因在内耳表达部位及荧光强度。结果经蜗窗膜注入3种病毒载体能有效转染内耳多种类型细胞,相对其他两种病毒载体,5型-腺病毒载体高效转染螺旋神经节及其神经纤维,持续稳定表达2周以上。结论不同病毒载体经蜗窗膜导入后,在耳蜗内的表达分布存在明显差异,5型-腺病毒高效转染耳蜗神经,可作为未来听神经病变分子治疗的载体工具选用。
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| 关 键 词： | 动物，实验 大鼠 圆窗 病毒蛋白质类 |
| 收稿时间： | 2008-08-19 |
Comparative study of fluorescent distribution within the rat cochlea after three viral vectors inoculation in round window membrane |
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| LEI Li,HAN Demin ,Ministry of Education,Beijing,China). Comparative study of fluorescent distribution within the rat cochlea after three viral vectors inoculation in round window membrane[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2009, 16(11): 608-610 |
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| Authors: | LEI Li HAN Demin Ministry of Education Beijing China) |
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| Affiliation: | LEI Li,HAN Demin (Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Key Laboratory of Otolaryngology Head and Neck Surgery(Capital Medical University),Ministry of Education,Beijing,100730,China) |
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| Abstract: | OBJECTIVE To determine the most effective viral vector in infecting the spiral ganglion cells and cochlear nerve fibers among three viral vectors that was inoculated via round window membrane. METHODS Three types vectors carrying GFP gene including Lentivirus, Adenovirus-5 and Adenoassociated virus-2 were infused into the perilymphatic space through the round window membrane with micro injector in rat respectively. The distribution and intensity of green fluorescence were estimated by fluorescent microscope... |
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| Keywords: | Animal Experimentation Rats Round Window Viral Proteins |
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