酚妥拉明和地塞米松对吸入性损伤大鼠肝脏损伤的保护作用及机理

目的　观察酚妥拉明和地塞米松对重度吸入性损伤大鼠肝脏损害的保护作用及机制。方法　将 72只大鼠随机分为A、B、C 3组。对照组 (C组 )腹腔注射生理盐水 ,A组腹腔注射酚妥拉明 ,B组腹腔注射地塞米松。另取 6只非致伤大鼠做伤前值测定 ,再取 6只致伤大鼠 ,伤后 30分钟做伤后值测定。结果　C组谷丙转氨酶 (GPT)、总胆红素 (TBIL)、丙二醛 (MDA)、MDA/超氧化物歧化酶 (SOD)及组织含水量 (WCT)均明显增高 ,组织中一氧化氮 (NO)水平较伤前值变化不大 ,SOD、ATP水平降低。A组和B组中TBIL、GPT、MDA、MDA/SOD、NO明显低于C组 (P <0 .0 1 ) ,A组ATP和SOD明显高于C组 (P <0 .0 1 ) ;B组SOD高于C组 (P<0 .0 1 ) ,B组的ATP则与C组差别不显著 (P >0 .0 5 ) ,B组肝组织含水量低于对照组 (P <0 .0 1 )。结论　酚妥拉明与地塞米松均可能通过调节机体的NO水平进而提高机体抗氧化能力和能量代谢 ,从而对重度吸入性损伤后大鼠的肝脏起保护作用。在改善能量供应和降低MDA方面酚妥拉明效果优于地塞米松

Effect of phentolamine and dexamethasone on liver injury in rats with severe smoke inhalation injury

Objective To observe the protective effect of phentolamine and dexamethasone on liver injury in rats with severe smoke inhalation injury,and to explore their potential mechanism.Methods Seventy two rats were divided into three groups: control group(saline treatment), phentolamine treatment group(group A),and dexamethasone treatment group(group B). In addition,6 animals without inhalation injury served as normal controls,and another 6 animals subjected to inhalation injury were sacrificed at 30 minutes.Results In the control group,levels of total bilirubin(TBIL),glutamic pyruvic transaminase enzyme (GPT),malondialdehyde(MDA),MDA/superoxide dismutase (SOD),and tissue water content increased,and plasma NO levels increased while liver SOD,ATP contents obviously decreased. TBIL,GPT,MDA,MDA/SOD and plasma and tissue NO level in both group A and group B were markedly lower than those in control group(P<0.01). Contents of SOD and ATP in group A was higher than control group. SOD of group B was higher than group C(P<0.01),but the ATP content of group B was not higher than group C(P>0.05). Tissue water content of group B was lower than group C(P<0.01).Conclusion Both phentometabolism, which may be associated with the protective effect on the renal function increased in rats with severe smoke inhalation injury.