RhoC基因高表达对胃癌细胞血管内皮细胞生长因子、碱性成纤维细胞生长因子表达的影响

目的 探讨RhoC基因对胃癌细胞血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)表达的影响及其意义。方法 常规脂质体转染法将RhoC基因重组质粒转染人胃癌细胞系SGC7901；应用免疫细胞化学链霉菌抗生物素蛋白-过氧化酶(SP)法检测胃癌细胞转染RhoC基因重组质粒前后VEGF、bFGF蛋白表达的变化。结果 转染RhoC基因重组质粒在SGC7901细胞中有稳定表达。转染RhoC基因重组质粒SGC7901表达VEGF、bFGF明显强于对照组。结论 体外条件下RhoC基因可促进胃癌细胞VEGF、bFGF的表达。这可能是RhoC基因促进胃癌细胞侵袭、转移的分子基础。RhoC基因的表达可望作为估计胃癌转移的参考指标。

Influence of the overexpression of RhoC gene on the expression of vascular endothelial growth factor and basic fibroblast growth factor in human gastric cancer cell line

Objective To explore the influence of RhoC gene on the expression of vascular en- dothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in human gastric cancer cell line. Methods The recombinant vector (pcDNA 3.1-RhoC) was transfected into SGC7901 cells with LIPFECTAMINE~(TM)Reagent. The expression of VEGF and bFGF was detected by the immunohistoche- mical stain in SGC7901 cells. Results The recmbinant vector (pcDNA 3.1-RhoC) was expressed steadi- ly in SGC7901 cells. The expression of VEGF and bFGF in the pcDNA 3.1-RhoC-transfected SGC7901 cells was significantly stronger than that of the pcDNA 3.1-transfected SGC7901 cells and untransfected SGC7901 cells. Conclusion RhoC gene could increase the expression of VEGF and bFGF in human gas- tric cancer cell line in vitro, which might be contributed to the molecular foundation of RhoC gene en- hancing invasiveness and metastasis of human gastric cancer cell line. The expression of RhoC gene may act as a good marker for the metastasis of gastric cancer.