| 人肝癌组织特异性γ-谷氨酰转移酶分离纯化 |
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| 引用本文： | 王念跃,赵伟,李勇,端木浩,黄宇峰. 人肝癌组织特异性γ-谷氨酰转移酶分离纯化[J]. 陕西医学杂志, 2007, 36(7): 799-801 |
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| 作者姓名： | 王念跃 赵伟 李勇 端木浩 黄宇峰 |
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| 作者单位： | 1. 东南大学医学院附属南京第二医院,南京,210003
2. 南京军区南京总医院全军医学检验中心 |
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| 基金项目： | 江苏省南京市医学科技发展计划 |
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| 摘 要： | 目的:分离纯化人肝癌组织中特异性γ-谷氨酰转移酶(GGT),为进一步制备其抗血清提供抗原材料,以便对肝癌早期诊断进行研究。方法:人肝癌组织经匀浆、离心、溶解、透析、凝胶层析及凝集素亲和层析等步骤进行分离纯化;纯化过程用IFCC推荐方法监测GGT催化活性,紫外法监测蛋白质出峰图谱,Lowry法测定蛋白含量。结果:经过粗提、DEAE阴离子交换层析、凝胶过滤层析及凝集素亲和层析等纯化步骤得到电泳纯的GGT,其比活为10.58U/mg蛋白,纯化倍数为84.9,得率为1.60%。结论:该方法能够较好地分离纯化人肝癌组织中特异性GGT。
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| 关 键 词： | 肝肿瘤,实验性/酶学 γ-谷氨酰转移酶/分离和提纯 色谱法,凝胶/方法 色谱法,亲和/方法 |
| 修稿时间： | 2006-07-17 |
Purification of special γ-glutamyltraspeptidese in human liver cancer tissue |
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| Wang Nianyue Zhao Wei Li Yong et al. Purification of special γ-glutamyltraspeptidese in human liver cancer tissue[J]. Shaanxi Medical Journal, 2007, 36(7): 799-801 |
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| Authors: | Wang Nianyue Zhao Wei Li Yong et al |
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| Affiliation: | Nanjing Second Hospital Affiliated to Southeast University Medical College,Nanjing 210003 |
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| Abstract: | Objective:To Purify special γ-glutamyltraspeptidese from human liver cancer tissue, which is then used to produce antibodies targeted for early diagnosis of liver cancer. Methods:Human liver cancer tissue is purified by rough extraction, dialyzation, ion-exchange chromatography and lectin affinity chromatography etc. Results:The special GGT in human liver cancer tissue was purified. The catalysis units per milligram protein of GGT have been increased about 84.9 times. And the yield of the GGT was 1.60%. Conclusion:This protocol is efficient to purity GGT from human liver cancer tissue. |
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| Keywords: | Liver neoplasms experimental/enzymology gamma-glutamylctransferase/isoation & purification Chromatography gel/methods Chromatography affinity/methods |
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