白藜芦醇对恶性黑色素瘤生长抑制作用的体外及体内研究

目的:研究白藜芦醇在体外和体内对恶性黑色素瘤的抗肿瘤效果.方法:采用MTT法测定白藜芦醇对小鼠B16及人A375细胞的增殖抑制率;用Western blot方法检测白藜芦醇对B16细胞中p-Akt蛋白表达的影响;建立B16皮下种植瘤小鼠模型,观察不同剂量的白藜芦醇对小鼠皮下瘤的生长抑制作用.结果:体外实验发现在白藜芦醇对B16及A375细胞均显示了良好的浓度依赖性;Western blot显示了不M浓度的白藜芦醇有效的抑制了p-Akt蛋白的表达,提示其抗肿瘤机制可能与此有关;体内实验证实不同浓度的白藜芦醇对恶性黑色素瘤的生长产生了明显的抑制作用.结论:体外和体内实验证实白藜芦醇有效的抑制了恶性黑色素瘤的生长,并且发现其抗肿瘤机制可能通过抑制p-Akt蛋白的表达来实现.这为白藜芦醇在恶性黑色素瘤的临床治疗的可能应用提供了实验基础,也为我国中草药的抗肿瘤效果的开发提供了具体思路.

In vitro and in vivo evaluation of antitumor effect of resveratrol against malignant melanoma

Objective:To explore the antitumor effect of resveratrol against malignant melanoma in vitro and in vivo. Methods:Murine B16 and human A375 cells were cultured in vitro and the growth inhibition effects of resveratrol were detected by MTT method. After the pretreatment with different doses of resveratrol,cell protein was extracted and the expression levels of protein p-Akt were detected by Western blot. B16 cells transplant model was established in male ICR mice and the tumor growth inhibition effects induced by resveratrol were detected by measuring tumor volume every two days. Results:Cytotoxicity test showed that cell growth was significantly inhibited by resveratrol. Moreover,resveratrol effectively downregulated the expression of protein p-Akt. Animal experiments also demonstrated that resveratrol efficiently inhibited tumor growth without obvious bodyweight change. Conclusion:resveratrol was demonstrated as a potential anti-melanoma agent both in vitro and in vivo with the possible mechanism of downregulation of p-Akt expression. The current study provided the experimental basis for the clinical application of resveratrol against malignant melanoma and also offered a detailed strategy for developing the antitumon effect of Chinese herbal medicines.