金黄色葡萄球菌肠毒素的检测及结果分析

目的了解引起临床感染的金黄色葡萄球菌(SA)携带肠毒素(SE)的情况。方法对从临床标本中分离出的260株SA,用反向间接血凝试验检测其肠毒素,耐甲氧西林金黄色葡萄球菌(MRSA)检测按2005年美国临床实验室标准化委员会(NCCLS)推荐的检测头孢西丁方法进行。结果260株SA中有172株携带肠毒素,占66.2%,主要为SEA型10.8%(28/260)、SEB型14.6%(38/260)、SEC型10.0%(26/260),同时携带二种或二种以上肠毒素的占23.8%(62/260);其中MRSA134株,全部携带肠毒素,且以SEA、SEB、SEC为主,分别为19.4%、16.4%、13.4%;甲氧西林敏感金黄色葡萄球菌(MSSA)126株,携带肠毒素率为30.0%(38/126),以SEB为主,占9.5%。结论临床上SA分离株大部分都携带肠毒素,且以SEA、SEB、SEC为主,MRSA的带毒率高于MSSA。

Detetive analysis of Staphylococcus aureus enterotoxin

Objective To survey the distribution of enterotoxin in staphylococcus aureus(SA)isolated from clinical specimens.Methods enterotoxin of Staphylococcus aureus were detected by reverse passive hemagglutination(RPHA)test.Methicllin resistant Staphylococcus aureus(MRSA)were detected according to the recommended method of NCCLS.Results Staphylococcus aureus with enterotoxin was 66.2%(172/260),Most of them were SEA 10.8%(28/260),SEB 14.6%(38/260),SEC 10.0%(26/260)respectively.The isolates with more than one enterotoxin was 23.8%(62/260),134 MRSA isolates were all with enterotoxin and with 19.4% of SEA,16.4% of SEB,13.4% of SEC respectively,126 MSSA isolates were 38 strains with enterotoxin.Conclusions Most of SA isolated from clinical specimens are the types of SEA,SEB,SEC.The rate of MRSA with enterotoxin is higher than that of MSSA.