Is chemotaxis a factor in the migration of precardiac mesoderm in the chick? |
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Authors: | H S Easton R Bellairs and J W Lash |
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Institution: | (1) Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT London, United Kingdom;(2) Department of Anatomy, University of Pennsylvania, 19104-6058 Philadelphia, PA, USA |
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Abstract: | Summary The chick heart is formed from bilateral patches of presumptive cardiac mesoderm cells which migrate over the endoderm and
fuse in the midline. We have tested the possibility that this migration is controlled, at least in part, by a chemotactic
substance exuded by the anterior end of the endoderm. We have used chick/quail combinations to follow naturally marked cells
during the course of their migration. Chimaeric embryos were formed by fusing together parts of chick and quail embryos of
stage 5–6. Each embryo possessed two pairs of precardiac regions, the quail pair lying immediately anterior to that of the
chick. These chimaeras were then explanted in embryo culture. In the event of chemotaxis, cells from the posterior end of
the quail precardiac mesoderm might be expected to invade the chick area. Samples of explants and chimaeras were examined
at intervals from 2 to 24 h, but in no case were cells found to have changed their direction of migration as a result of the
proximity of anterior endoderm. It is concluded that this work does not provide evidence for a chemotactic attraction by the
anterior end of the endoderm.
Supported by the following grants: NIH HD 21048, HD 06819, and AHA 880696 (JWL); the British Heart Foundation, and Action
Research (R.B.); and an SERC postgraduate studentship (HSE). |
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Keywords: | Fibronectin Precardiac mesoderm Cell migration Chemo Chick/quail |
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