海藻酸钠对酪氨酸酶的抑制作用

目的 研究海藻酸钠对酪氨酸酶及黑色素生成的抑制作用方法 开展酶抑制动力学实验，通过荧光光谱分析海藻酸钠对酪氨酸酶活性抑制效果，并研究海藻酸钠对黑素细胞抑制效果结果 海藻酸钠能够显著抑制酪氨酸酶活性，海藻酸钠浓度小于20 mmol/L时，浓度越高，抑制效果越好。超过20 mmol/L时， 海藻酸钠对酪氨酸酶活性抑制率65.45%，海藻酸钠浓度增加，对胞内黑色素生成抑制率随之上升，降低黑色素生成量；海藻酸钠与酪氨酸酶的相互作用对氨基酸残基疏水性环境产生破坏性，出现内源性荧光光谱红移；海藻酸钠对黑素细胞细胞活性超过90%，未产生明显毒性结论 皮肤相关疾病治疗药剂及美白化妆产品中可以使用海藻酸钠，效果良好。

Inhibitory Effect of Sodium Alginate on Tyrosinase

Objective To study the inhibitory effect of sodium alginate on tyrosinase and melanin production. Methods Enzyme inhibition kinetics experiment was carried out. The inhibitory effect of sodium alginate on tyrosinase activity was analyzed by fluorescence spectroscopy, and the inhibitory effect of sodium alginate on melanocytes was studied. Results Sodium alginate could significantly inhibit tyrosinase activity. When the concentration of sodium alginate was less than 20 mmol/L, the higher the concentration, the better the inhibitory effect. More than 20 mmol/L, the inhibition rate of sodium alginate on tyrosinase activity was 65.45%. With the increase of sodium alginate concentration, the inhibition rate of intracellular melanin production increased, and the melanin production decreased. The interaction between sodium alginate and tyrosinase was destructive to the hydrophobic environment of amino acid residues, resulting in the red shift of endogenous fluorescence spectra. Sodium alginate had no obvious toxicity to melanocytes with the activity of more than 90%. Conclusion Sodium alginate can be used in the treatment of skinrelated diseases and whitening cosmetics, and the effect is good.