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肝癌及癌旁组织中端粒酶检测的临床意义
引用本文:傅建民,张伟,金顺钱.肝癌及癌旁组织中端粒酶检测的临床意义[J].中华肿瘤杂志,1998(6).
作者姓名:傅建民  张伟  金顺钱
作者单位:中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所腹部外科
基金项目:中国医学科学院CMB院校长基金
摘    要:目的研究端粒酶作为原发性肝细胞癌(HCC)肿瘤标志物的可能性。方法采用TRAP方法检测了33例原发性肝细胞癌及其33例癌旁组织、4例肝转移癌及其4例癌旁组织、6例肝良性肿瘤和6例正常肝组织中的端粒酶活性。结果33例原发性肝细胞癌组织中,有30例端粒酶表达阳性,其阳性率为90.9%。33例癌旁组织中,有9例端粒酶表达阳性,其阳性率为27.3%。4例肝转移癌端粒酶活性均阳性,4例癌旁组织中,2例端粒酶表达阳性。6例肝良性肿瘤中,仅1例端粒酶表达阳性。6例正常肝组织端粒酶表达均阴性。肝癌组织端粒酶表达与肿瘤临床病理特征无关。结论肝癌组织中普遍存在端粒酶活性表达,而良性和正常肝组织中端粒酶活性较少表达。端粒酶有可能成为诊断原发性肝细胞癌的肿瘤标志物

关 键 词:肝肿瘤  癌.肝细胞  端粒酶  聚合酶链反应

Telomerase activity in liver cancer and the adjacent tissue
Fu Jianmin,Zhang Wei,Jin Shunqian,et al..Telomerase activity in liver cancer and the adjacent tissue[J].Chinese Journal of Oncology,1998(6).
Authors:Fu Jianmin  Zhang Wei  Jin Shunqian  
Abstract:Objective To investigate the possibility of using telomerase as a tumor marker for human primary hepatocellular carcinomas (HCC). Methods Human liver specimens, comprising 33 HCC and adjacent peritumoral tissues, 4 hepatic metastases and the adjacent peritumoral tissues, 6 benign liver lesions and 6 normal liver tissues, were examined for telomerase activity by TRAP assay based on PCR. Results Thirty of 33 HCC and 9 of 33 adjacent tissue specimens were positive for telomerase activity with a positive rate of 90.9% and 27.3%, respectively. Four hepatic metastatic cancer and 2 adjacent tissues were positive. Telomerase activity was negative in 6 nomal liver tissues and 5 of 6 benign liver lesions. There was no correlation between the clinical stage of HCC and their telomerase activity. Conclusion Positive telomerase activity occurs in the majority of HCC examined. It can be used as a tumor marker in the diagnosis of HCC.
Keywords:Liver neoplasms    Carcinoma  hepatocellular    Telomerase    Polymerase chain reaction  
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