Heterogeneity of the antigenic centres of albumin toward chicken antibodies

Pooled chicken antiserum to bovine serum albumin contained antibodies capable of binding eighteen sites on the albumin molecule. Quantitative study of the reactivity of albumin following gradual coupling to tyrosyl, histidyl and lysyl residues, indicated that these residues are in close relationship to the antigenic centres, and that the centres and their respective antibodies are heterogeneous.