Prediction of conserved microRNAs from skin and mucosal human papillomaviruses |
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| Authors: | Gu Wenyi An Jiyuan Ye Ping Zhao Kong-Nan Antonsson Annika |
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| Affiliation: | (1) Diamantina Institute for Cancer, Immunology and Metabolic Medicine, Princess Alexandra Hospital, The University of Queensland, Woolloongabba, Brisbane, Australia;(2) The Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, Brisbane, Australia;(3) UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia;(4) Present address: Genetics and Population Health Division, Queensland Institute of Medical Research, Brisbane, QLD, Australia;(5) Present address: Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Rds (Bldg 75), The University of Queensland, Brisbane, QLD, 4072, Australia |
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| Abstract: | Eight human papillomavirus (HPV) types including four cutaneous HPV types (HPV-5, HPV-8, HPV-20 and HPV-38) and four mucosal HPV types (HPV-6, HPV-11, HPV-16 and HPV-18) were selected for this miRNA study. Pre-miRNAs were predicted using a computer programme, and the conserved mature miRNAs were compared to currently known miRNAs. Predicted HPV miRNAs related to miR-466, -467 and -669 were common and specific to the mucosal HPV types. Northern blot hybridization confirmed a predicted miRNA in HPV-positive cervical cancer cell lines encoded by mucosal HPVs. HPV-38 was predicted to express an miRNA conserved to human let-7a and the expression of let-7a, in HPV-38-positive non-melanoma skin cancer (NMSC) biopsies was 10-fold higher than those with HPV-positive (for other types except HPV-38) and HPV-negative NMSCs, suggesting that let-7a expression might be related to HPV-38 infection. Potential gene targets of the predicted miRNA that may aid HPV in infection and pathogenesis were also analysed. |
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