成年小鼠心肌细胞的分离及电生理特性

目的 :摸索成年小鼠心肌细胞分离方法并观察其电生理特性。方法 :采用酶消化法分离单个心肌细胞 ,应用膜片钳全细胞记录技术记录钙电流和钾电流。结果 :本法分离所得心肌细胞横纹清晰 ,一次性复钙 ,可获得 5 0 %～6 0 %的耐钙细胞 ;并具有正常电生理活性 ,易于形成高阻封接 ,可用于钙电流和钾电流记录。结论 :本实验所采用的分离方法经济、简便、成功率高 ,所获细胞具有正常的电生理活性

Isolation and electrophysiological characteristics of cardiac myocytes in adult mice

AIM: To grope the mothod used to isolate adult mice cardiac myocytes and observe the electrophysiological characteristics of the myocytes. METHODS: Enzyme digestion method was used to isolate single cells and whole-cell patch clamp recording technique was used to record Ca 2+ and K + currents. RESULTS: Rod-shaped, clear-striped cardiac myocytes with normal electrophysiological characteristics were gotten. After exposed to normal calcium concentration solution, 50%~60% calcium-resistant myocytes were left. Giga-ohm seal can be easily achieved and Ca 2+ and K + currents were successfully recorded. CONCLUSION: It is an economic, convenient and reliable method to isolate adult mice cardiac myocytes with normal electrophysiological characteristics.