阿加曲班对大鼠肢体缺血再灌注损伤肠系膜微循环影响的实验研究

目的探讨阿加曲班对大鼠肢体缺血再灌注损伤肠系膜微循环的影响，为缺血再灌注损伤的防治提供新的思路。方法采用大鼠肢体缺血再灌注模型，将14只Wistar大鼠随机分为每组7只：对照组和阿加曲班组，每组7只。2组大鼠均于再灌注2h后，酶联免疫吸附（ELISA）法测定血浆P-选择素、细胞间粘附分子-1（ICAM-1）浓度；应用BI-2000医学图像分析系统观察和记录肠系膜微循环改变，指标包括肠系膜微动脉管径（AD）和微静脉管径（VD）、肠系膜微动脉血流速度（AFV）和微静脉血流速度（VFV）以及白细胞粘附情况。结果大鼠肢体缺血再灌注2h后，阿加曲班组血浆P-选择素浓度、血浆ICAM—1浓度显著低于对照组（P〉0．05）。与对照组相比，阿加曲班组肠系膜微循环障碍较轻，AD和VD与对照组之间差异无统计学意义（P〉0．05），AFV和VFV明显高于对照组（P〈0．05），白细胞粘附较对照组轻（P〈0．05）。结论阿加曲班能够降低血浆P-选择素和ICAM-1浓度，进而改善大鼠肢体缺血再灌注损伤时的肠系膜微循环障碍．

The effects of argatroban on mesentery microcirculation in rats with limb ischemia/reperfusion

Objective To observe the effects of argatroban on mesentery microcirculation in rats with limb isehemia/reperfusion injury in order to provide a new way for the prevention and treatment of ischemia/reperfusion injury. Methods 14 Wistar rats were randomly divided into control group and agatroban group, 7 rats in each group. The rat models of limb ischemia/reperfusion injury were established. The levels of plasma P-selectin and plasma ICAM-1 were detected by enzyme linked immunosorbent assay（ELISA） at the point of 2h after reperfusion. The BI - 2000 medical analysis system was used to observe the changes of mesenteric microcirculation. The microvascular arteriole diameter （ AD ）, microvascular venule diameter （ VD ）, arteriole flow velocity （ AFV ）, venule flow velocity （VFV） and the number of adhesive leukocytes were detected. Results After 2-hour reperfusion,the levels of plasma P-selectin and plasma ICAM-1 in agatroban group were significantly lower than those in control group （ P 〈 0.05）. As compared with control group, mesenteric microcirculation disturbance in agatroban group was lighter, however, there was no significant difference in AD, VD between agatroban group and control group （ P 〉 O. 05 ）. The AFV and VFV in agatroban group were significantly higher than those in control group （P 〈 O. 05 ） , and the leukocyte adhesion in agatroban group was lighter than that in control group （P 〈 0.05 ） . Conclusion Agatroban can reduce the concentrations of plasma P-selectin and plasma ICAM-1 so as to improve mesenteric microcirculation disturbance in limb ischemia/reperfusion injury of rats.