子宫颈组织中人乳头瘤病毒16型E6基因片段的定量PCR检测

目的探讨子宫颈组织中人乳头瘤病毒(HPV)16型E6基因的含量与疾病严重程度的关系.方法用定量聚合酶链反应(PCR)检测20例慢性宫颈炎,6例宫颈非典型增生,18例宫颈癌组织中HPV16E6基因的拷贝数.结果HPV16E6基因在慢性宫颈炎,宫颈非典型增生及宫颈癌组织中的平均拷贝数(拷贝/μgDNA)分别为6.16×104,5.33×106和6.45×106.统计学处理表明,宫颈非典型增生及宫颈癌组织中E6基因的拷贝数显著高于慢性宫颈炎(P<0.01),宫颈癌组织中E6基因的拷贝数高于宫颈非典型增生组织,但差异无显著性(P>0.05).结论HPV16E6基因的拷贝数与宫颈疾病程度呈正相关,定量检测HPV16E6基因可作为监测宫颈癌高危人群的一种方法.

Detection of HPV16 E6 gene in cervical tissues by quantitative polymerase chain reaction

OBJECTIVE: To study the relationship between the quantities of HPV16 E6 gene in cervical tissues and the course of cervical disease. METHODS: The number of copies of HPV16 E6 gene was detected by competitive PCR in 44 positive samples including 20 cases of chronic cervicitis, 6 cases of cervical dysplasia and 18 cases of cervical cancer. RESULTS: The mean copies of HPV16 E6 gene in chronic cervicitis, cervical dysplasia and cervical cancer were 6.16, 5.33, and 6.45 copies in each microgram of cervical tissue respectively. The quantities of HPV16 E6 gene were not significantly different between cervical dysplasia and chronic cervicitis (P<0.01). CONCLUSIONS: This study underscores the importance of the relationship between the quantities of HPV16 E6 gene and the course of cervical disease. It also suggests that quantification of HPV16 E6 gene may be useful as a prognostic tool to identify women who are at increased risk of developing cervical cancer.