广东主要柑桔果实过氧化物酶活性及其同工酶谱

采用蕉柑(Citrus reticulata cv.tankan Hayata)为材料测定了果皮中过氧化物酶的特性反应,可溶态和结合态不同状态酶的分配及果实中不同部位酶的分布,确定果顶的有色皮层为检测柑桔果实过氧化物酶的取样点。比较广东五个主要柑桔品种可溶态过氧化物酶活性的结果表明,酶活性与果实耐藏性呈负相关。蕉柑原产广东,是甜橙与柑的自然杂交种。从椪柑、蕉柑和暗柳橙的过氧化物酶同工酶酶谱初步判断,三者之间有一定亲缘关系。还着重对广东柑桔果实中过氧化物酶的分布与果汁加工质量的关系进行了讨论。

AN EXPERIMENT ON THE PEROXIDASE ACTIVITY AND ISOENZYME PATHERNS OF MAJOR CITRUS FRUITS GROWING IN GUANGDONG

This experiment was to assay the proper reaction of citrus perozidase in the ezocarp of Tankan mandarin (Citus reticulata cv. tankan Hayata) , the proportion of the bound enzyme to the soluble enzyme as well as the distribution of citrus peroxidase in the fruit were also investigated. Samples for this experiment were taken from the flavedo (the colored portion of the exocarp) to test the peroxidase of citrus fruits. A comparisom was made to determine the soluble peroxidase activity among five varieties of citrus fruits growing in Guangdong. The results showed that the correlation between peroxidase activity and storage life of fruits was negative. Tankan mandarin was known as an endemic variety growing in Swatow, Guangdong, because it was a natural hybrid between tangerine and Sweet orange. As shown by the isoenzyme patterns, Tankan, Ponkan and Anliu Sweet Orange, all these three varieties were considered to have cartain filial affinity. Also the relationship between the distribhutiong of peroxidase in the citrus fruits and the fruit juise quality during processing was discussed particularly.