| 溴百里酚蓝分光光度法测定人血清白蛋白 |
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| 引用本文： | 翟海云,高建华,陈彬.溴百里酚蓝分光光度法测定人血清白蛋白[J].郑州大学学报(医学版),2002,37(1):28-30. |
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| 作者姓名： | 翟海云 高建华 陈彬 |
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| 作者单位： | 1. 郑州大学化学系,郑州,450052
2. 复旦大学化学系,上海,200433 |
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| 基金项目： | 河南省教委自然科学基金资助项目98150007 |
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| 摘 要： | 目的：建立一种测定蛋白质的新方法。方法：利用溴百里酚蓝与蛋白质的结合反应，在615nm波长处测定体系的吸光度。结果：试验的线性范围为11.3-800mg/L，回收率为95.0%-105.6％。平行样品的相对标准偏差为3.28%，工作曲线线性良好，回归系数为r=0.9980。结论：方法简单，准确快速，线性范围较宽，重现性好。
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| 关 键 词： | 溴百里酚蓝 分光光度法 蛋白质 血清诊断 白蛋白 |
| 修稿时间： | 2001年6月12日 |
Determination of human serum albumin with the use of bromthymol blue spectrophotometry |
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| ZHAI Haiyun,GAO Jianhua,CHEN Bin.Determination of human serum albumin with the use of bromthymol blue spectrophotometry[J].Journal of Zhengzhou University: Med Sci,2002,37(1):28-30. |
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| Authors: | ZHAI Haiyun GAO Jianhua CHEN Bin |
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| Institution: | ZHAI Haiyun,GAO Jianhua,CHEN BinDepartment of Chemistry,Zhengzhou University,Zhengzhou 450052 Department of Chemistry,FudanUniversity,Shanghai 200433 |
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| Abstract: | Aim: To establish a new method to determine protein. Methods: Through the reaction between protein and bromthymol blue, the absorption of the system was measured at 615 nm. Results: The linear range was 11.3-800.0 mg/L, the recovery ratio was 95.0% - 105.6% . The relative standard deviation of parallel sample was 3.28% , the standard curve had better linear characteristic and the regression coefficient of the curve was 0.998 0. Conclusion: The method is simple, accurate, and quick with a wider linear range and a better repeatability. |
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| Keywords: | bromthymol blue spectrophotometry protein |
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