改善微囊化人视网膜色素上皮细胞生长状态的研究

目的 观察上皮细胞培养液中添加果糖、成纤维细胞生长因子和牛磺酸后对人视网膜色素上皮(hRPE)细胞海藻酸钠-壳聚糖-海藻酸钠微囊化后的细胞总数、存活率及活细胞数的影响.方法 将hRPE细胞微囊化后将其分为4组,一组作为空白对照组(空白组)只加入上皮细胞培养液、另外3组在培养液中分别加入果糖(果糖组)、成纤维细胞生长因子(成纤维细胞生长因子组)、牛磺酸(牛磺酸组)后培养7 d,在0、1、3、7 d测定微囊内细胞的细胞总数、存活率及活细胞数.结果 4组细胞微囊化后的hRPE细胞至培养7 d,细胞存活率最低为(75.00±3.00)%,但各组间差异无统计学意义(P均＞0.05);而微囊内细胞总数牛磺酸组(7.20±0.36)×104、成纤维细胞生长因子组(8.00±0.46)×104,比空白对照组(6.10±0.56)×104明显增多(t值分别为-2.872、-4.564,P均＜0.05),果糖组(6.00±0.46)×104与空白组比较差异无统计学意义(P＞0.05).结论 细胞生长因子、牛磺酸可促进海藻酸钠-壳聚糖-海藻酸钠微囊内的hRPE细胞的增殖.

Abstract：

Objective To observe the effects of fructose,fibroblast growth supplement(FGS) and ethylamine sulfonic acid on the total number,the survival rate and the survival number of Human Retinal Pigment Epithelial(hRPE) Cell.Methods Microencapsulated hRPE cells were plated and cultured in four kinds of mediums,which contained fructose,fibroblast growth supplement(FGS),ethylamine sulfonic acid or no extra ingredient respectively.The total cell number,survival rate and viable cell number of the microencapsulated hRPE cell on day 0th,1st,3rd,7th were calculated.Results After 7days of culture,the lowest cell survival rate of microencapsulated hRPE cells in the four groups was(75.00±3.00)%,but there were no significantly differences(Ps＞0.05) among the groups.The total number of cells in the fibroblast growth factor group([8.00±0.46]×104) and ethylamine sulfonic acid group([7.20±0.36]×104) were significantly higher than the blank group(([6.10±0.56]×104),Ps＜0.05),while no statistical difference was observed in the comparison between the fructose group([6.00±0.46]×104) and blank control(P＞0.05).Conclusion The FGS and ethylamine sulfonic acid can promote the proliferation of the microencapsulated hRPE cells.

A Study on improving growing status of microencapsulated human retinal pigment epithelial cell

Objective To observe the effects of fructose,fibroblast growth supplement(FGS) and ethylamine sulfonic acid on the total number,the survival rate and the survival number of Human Retinal Pigment Epithelial(hRPE) Cell.Methods Microencapsulated hRPE cells were plated and cultured in four kinds of mediums,which contained fructose,fibroblast growth supplement(FGS),ethylamine sulfonic acid or no extra ingredient respectively.The total cell number,survival rate and viable cell number of the microencapsulated hRPE cell on day 0th,1st,3rd,7th were calculated.Results After 7days of culture,the lowest cell survival rate of microencapsulated hRPE cells in the four groups was(75.00±3.00)%,but there were no significantly differences(Ps＞0.05) among the groups.The total number of cells in the fibroblast growth factor group([8.00±0.46]×104) and ethylamine sulfonic acid group([7.20±0.36]×104) were significantly higher than the blank group(([6.10±0.56]×104),Ps＜0.05),while no statistical difference was observed in the comparison between the fructose group([6.00±0.46]×104) and blank control(P＞0.05).Conclusion The FGS and ethylamine sulfonic acid can promote the proliferation of the microencapsulated hRPE cells.