增殖细胞核抗原在子宫内膜病变组织中的表达及临床意义

目的:研究增殖细胞核抗原(PCNA)在子宫内膜病变中的表达规律及其在子宫内膜癌的发生、发展及估计患者预后中的临床价值。方法:用免疫组化SP法检测子宫内膜增殖症30例、非典型增生30例、子宫内膜癌90例,共150例作为实验组,分泌期及增生期内膜各30例作为对照组,观察PCNA在不同内膜病变及正常内膜中的阳性表达率。结果:PCNA在正常子宫内膜、子宫内膜增殖症、非典型增生阳性表达率依次呈升高趋势,子宫内膜增殖症与增生期内膜比较差异无统计学意义,P>0.05。而在非典型增生内膜和内膜癌组织中,PCNA表达明显增高,显著高于子宫内膜增殖症和增生期内膜,差异有统计学意义,P<0.01;且PCNA的阳性率与临床分期、细胞分化程度及淋巴转移有关。结论:PCNA与子宫内膜癌的发生和发展密切相关,PCNA表达对于子宫内膜癌的诊断和判断预后有重要意义。

Expression of PCNA in endometrial diseases and clinical significance

OBJECTIVE: To investigate the expression of proliferating cell nuclear antigen(PCNA) in endometrial diseases and its clinical significance for the incidence of endometrial carcinoma and predicting prognosis. METHODS:The experimental group included 150 cases including 90 of endometrial carcinoma, 30 of edometrial hyperlpasia, 30 of atypical proliferation;the control group included 60 cases including 30 of proliferative endometrium and 30 of secretory endometrium. The expression of PCNA in these cases was deteded with the immunohistochemistry technology. RESULTS:There was a graduate increasing of the positive rate of PCNA expression in normal endometrium, endometrial hypwrplasia and atypical endometrium, and there was no statistical significance between endometrial hyperplasia and proliferative endometrium,P>0.05. In endometrial carcinoma, the expression increased significantly, which is higher than benign diseases and control group prominently,P<0.01.Additionally, the positive rate of PCNA increased with the increasing of clinical phase and grade of cellular differentiation. CONCLUSIONS: PCNA is closely related to the development and the progression of endometrial carcinoma. The expression of PCNA is of great value in predicting the prognosis of endometrial carcinoma.