罗勒胶囊对大鼠移植性肝癌经肝动脉化疗栓塞术后 骨桥蛋白和诱导性一氧化氮合酶表达的影响

目的:观察罗勒胶囊对大鼠移植性肝癌经肝动脉化疗栓塞术(TACE)后细胞中骨桥蛋白(OPN)和诱导性一氧化氮合酶(iNOS)表达的影响,探讨罗勒胶囊对肝肿瘤乏氧微环境的影响及意义。方法:建立大鼠移植性肝癌模型,10 d后将造模成功的大鼠随机分为5组。对TACE+生理盐水组和TACE+罗勒胶囊高、中、低剂量组4组大鼠进行TACE,并分别给予生理盐水10 mL.kg-1.d-1和罗勒胶囊300,150,75 mg.kg-1.d-1ig,对非TACE对照组大鼠只进行开腹操作,不进行TACE。给药10d后取出移植性肝癌病理标本,免疫组化法检测肿瘤组织中OPN和iNOS表达情况。结果:瘤重:行TACE的4组大鼠与非TACE组比较均明显减轻(P<0.05),TACE+罗勒胶囊高剂量组较TACE+生理盐水组显著减轻(P<0.05);OPN和iNOS阳性表达率:TACE+生理盐水组与非TACE组比均显著升高(P<0.01);TACE+罗勒胶囊高、中剂量组较TACE+生理盐水组显著降低(P<0.01);TACE+罗勒胶囊高剂量组较TACE+罗勒胶囊中、低剂量组阳性表达率低(P<0.01)。结论:中药罗勒胶囊能改善肝肿瘤局部的乏氧微环境并促进细胞凋亡,使TACE后肿瘤组织中OPN和iNOS表达降低,抑制大鼠移植性肝癌TACE术后肿瘤的复发。

Effect of Basil Capsule on Expression of Osteopontin and Iinducible Nitric Oxide Synthase for Transplanted Liver Cancer in Ratafter Transcatheter Arterial Chemoembolization

Objective : To research the effect of Basil capsule on expression of osteopontin(OPN) and inducible nitric oxide synthase(iNOS) for transplanted liver cancer in rat after transcatheter arterial chemoembolization(TACE),and to discussion the effect of basil capsule on hypoxia environmental. Method :Ten days after transplanted rat liver cancer model established, the successful rats model were randomly divided into five groups:without TACE group(A);TACE+ Saline group(B); TACE+ high-dose Basil capsule group(C); TACE+middose Basil capsule group(D); TACE+ low-dose Basil capsule group(E). For B to simulate the heal of TACE and give normal saline 10 mL·kg^-1·d^-1.For C,D and E to carry out Basil capsules 300, 150, 75 mg·kg^-1·d^-1ig, A of rats only for abdominal operations, without TACE.Ten days after embolism, the rats were sacrificed and the tumors were taken out and treated with chemoembolization. To demonstrate the expression rate of OPN and iNOS. Result : The expression rate of OPN and iNOS on B were higher than A(P<0.01).The expression rate of OPN and iNOS on C and D were lower than B(P<0.01).The expression rate of OPN and iNOS between D and E were higher than C (P<0.01). Conclusion : Basil capsule can obviously improve hypoxia environmental in local of rat hepatoma and can depress the expression of OPN and iNOS, can inhibit rat liver transplant tumor recurrence after TACE.