| 一测多评法优选黄连最佳提取工艺 |
| |
| 引用本文： | 汪坤,张振凌,贾秀梅. 一测多评法优选黄连最佳提取工艺[J]. 中国实验方剂学杂志, 2011, 17(24): 9-11 |
| |
| 作者姓名： | 汪坤 张振凌 贾秀梅 |
| |
| 作者单位： | 河南中医学院,郑州,450008 |
| |
| 基金项目： | 中医药行业科研专项(201007010) |
| |
| 摘 要： | 目的:优选黄连的最佳提取工艺。方法:应用一测多评法测定黄连中表小檗碱、黄连碱、巴马汀、小檗碱的含量,通过正交试验设计,以黄连中表小檗碱、黄连碱、巴马汀及小檗碱含量之和为指标优选黄连最佳提取工艺。结果:最佳工艺条件为10倍量70%乙醇提取3次,每次1.5 h。结论:该提取工艺可以有效提取黄连中小檗碱等多种生物碱。
|
| 关 键 词： | 一测多评法 黄连 提取工艺 高效液相色谱法 生物碱 |
| 收稿时间： | 2011-07-24 |
Optimization of Extraction Technology of Coptis chinensis with Multi-components Quantitation by One Marker |
| |
| WANG Kun,ZHANG Zhen-ling and JIA Xiu-mei. Optimization of Extraction Technology of Coptis chinensis with Multi-components Quantitation by One Marker[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(24): 9-11 |
| |
| Authors: | WANG Kun ZHANG Zhen-ling JIA Xiu-mei |
| |
| Affiliation: | Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China |
| |
| Abstract: | Objective :To optimize extraction technology of Coptis chinensis. Method :Determined the content of epiberberine,coptisine,berberine and palmatine with multi-components quantitation by one marker,orthogonal design was used to optimize extraction technology of C. chinensis with total content of epiberberine,coptisine,berberine and palmatine. Result :The best extracting condition was as follows:extracted 3 times with 10 times the amount of 70% ethanol for 1.5 h each time. Conclusion : Optimum extraction technology was effective for extracting multiphasic alkaloid from C. chinensis. |
| |
| Keywords: | multi-components quantitation by one marker Coptis chinensis extraction technology HPLC alkaloid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
