| HPLC对不同品种菊花中绿原酸含量的测定 |
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| 引用本文： | 孙英英,崔永霞,刘伟.HPLC对不同品种菊花中绿原酸含量的测定[J].中国实验方剂学杂志,2011,17(24):83-84. |
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| 作者姓名： | 孙英英 崔永霞 刘伟 |
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| 作者单位： | 1. 河南中医学院第三附属医院,郑州,450000
2. 河南中医学院分析测试中心,郑州,450008 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目 (2003350178) |
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| 摘 要： | 目的:建立HPLC测定菊花中绿原酸含量的方法。方法:HPLC,Dikma Diamonsil C18色谱柱(4.6 mm×200 mm,5μm),流动相甲醇-磷酸二氢钠缓冲液(15.6～1 000 mL),加入磷酸使pH 2.7](20∶80),流速1.0 mL.min-1,检测波长328nm。结果:绿原酸的检测线性范围0.2～1.0 mg.L-1(r=0.999 9),平均加样回收率98.78%,RSD 0.97%(n=6)。结论:方法可作为控制菊花的质量。
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| 关 键 词： | 高效液相 菊花 绿原酸 含量测定 |
| 收稿时间： | 2011/7/19 0:00:00 |
Determination of Chlorogenic Acid from Various Species of Chrysanthemum morifolium by HPLC |
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| SUN Ying-ying,CUI Yong-xia and LIU Wei.Determination of Chlorogenic Acid from Various Species of Chrysanthemum morifolium by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(24):83-84. |
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| Authors: | SUN Ying-ying CUI Yong-xia and LIU Wei |
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| Institution: | Henan College of Traditional Chinese Medicine;Center of Analysis and Measurement of Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China;Center of Analysis and Measurement of Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China |
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| Abstract: | Objective: The method of HPLC was established for the quality control of Chrysanthemum morifolium. Method : The scopoletin was performed on a column C18 with a mobile phase of MeOH-NaH2PO3(15.6-1 000 mL,pH 2.7), flowrate was 1.0 mL·min-1, detected at 328 nm. Result : The calibration curve was linear(r=0.999 9) within the range of 0.2-1.0 mg·L-1 for chlorogenic acid. The average recovery was 98.78% and RSD is 0.97% (n=6) The method was simple and accurate. Conclusion : The method of high performance liquid chromatography can be used for the quality control of C. morifolium. |
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| Keywords: | HPLC Chrysanthemum morifolium chlorogenic acid content determination |
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