三化汤对脑缺血再灌注大鼠神经功能、 脑含水量及脑组织病理改变的影响

目的:观察三化汤对脑缺血再灌注大鼠神经功能、脑含水量及脑组织病理形态学的影响。方法:将大鼠随机分为假手术组、模型组、三化汤低、高剂量组(7.2,14.4 g.kg-1)ig、尼莫地平组(8.1 mg.kg-)1ig,每日1次,连续7 d,给药1周后采用线栓法制备脑缺血再灌注大鼠模型。缺血2 h再灌注24 h后按照Zealonga 5级法对大鼠神经受损症状评分;并取脑称量脑湿重和脑干重,计算脑含水量;HE染色分析脑组织病理学改变。结果:与假手术组比,模型组大鼠神经受损症状严重(P<0.01),脑含水量明显升高(P<0.01),光镜见神经细胞明显肿胀、变性、坏死、炎细胞浸润及血管扩张等病理改变;与模型组比,三化汤高剂量组大鼠神经受损症状明显减轻(P<0.05),大鼠脑组织含水量明显降低(P<0.01)。结论:三化汤对大鼠脑缺血再灌注损伤具有一定的保护作用。

The Effect of Sanhua Decoction on Neurological Function, Brain Water Content and Brain Morphology of Cerebral Ischemia-reperfusion in Rat

Objective :To observe the effect of sanhuatang on neurological function, brain edema and brain morphology of cerebral ischemia-reperfusion in rat. Method :The rats were randomly divided in sham operation group, model group,low dose group of sanhuatang,large dose group of sanhuatang and nimodipine group every group were medicated once each day and lasted for 7 days. The cerebral ischemia reperfusion model was established by blocking middle cerebral artery of rat with suture method prepare. The score of nervous symptoms in rats was made by Zealonga 5 class. The brain water content was calculated through weighing anhydrous brain and hydrous brain. The pathological changes of brain tissue was analyzed by HE staining. Result: In model group the neurological symptoms were severer than in the sham group(P<0.01),brain water content was significantly higher(P<0.01), swelling, denaturation, necrosis, inflammatory cell infiltration and vasodilator od never cells were observed. In large dose group neurological symptoms of rats was significantly relieved (P<0.05), brain water content were significantly reduced (P<0.01). Conclusion : Sanhua decocotiom can protect injure of cerebral ischemia reperfusion in rats.