| 桂枝茯苓丸抗大鼠肝纤维化作用及其机制研究 |
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| 引用本文： | 李季,叶军,薛冬英,张洁,陈蓓,孙琳,刘志勇,李小红. 桂枝茯苓丸抗大鼠肝纤维化作用及其机制研究[J]. 中国实验方剂学杂志, 2011, 17(24): 171-175 |
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| 作者姓名： | 李季 叶军 薛冬英 张洁 陈蓓 孙琳 刘志勇 李小红 |
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| 作者单位： | 上海中医药大学附属普陀医院感染科,上海,200062 |
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| 摘 要： | 目的:观察桂枝茯苓丸对大鼠肝纤维化的拮抗作用,并探讨可能的作用机制。方法:按照随机数字表将60只Wistar大鼠分为正常组8只(A组)和造模组52只,正常组sc予生理盐水3 mL.kg-1,造模组采用等量40%四氯化碳(carbontetrachloride,CCl4)复制肝纤维化模型,共6周。再随机将造模组大鼠分为模型组(B组)、桂枝茯苓丸低、中、高剂量组(C,D,E组)每组7只大鼠。A组与B组大鼠均予生理盐水9.0 mL.kg-1ig,C,D,E组分别给予桂枝茯苓丸0.45,0.9,1.8 g.kg-1.d-1 ig治疗,共4周,并在治疗期间继续使用CCl4维持。采用免疫组化(immunohistochemistry,IH)法及实时定量PCR(real-timequantitative PCR,Real-time PCR)法检测各组大鼠肝组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、转化生长因子β1(transforming growing factorβ1,TGF-β1)、结缔组织生长因子(connective tissue grower factor,CTGF)、Ⅰ型胶原(CollagenⅠ,C-Ⅰ)及Ⅲ型胶原(CollagenⅢ,C-Ⅲ)的蛋白及基因表达量。结果:模型组大鼠肝组织α-SMA,TGF-β1,CTGF,C-Ⅰ,C-Ⅲ蛋白和mRNA的表达较正常组显著增多(P<0.01),治疗组均能下调大鼠肝组织α-SMA,TGF-β1,CTGF,C-Ⅰ以及C-Ⅲ蛋白和mRNA的表达,其中以D组,E组减少α-SMA,C-Ⅰ,C-Ⅲ蛋白的表达明显(P<0.05或P<0.01),以E组降低TGF-β1,CTGF蛋白表达最为显著(P<0.05),以D组,E组降低α-SMA,TGF-β1,CTGF mRNA的表达显著(P<0.01),以E组减少C-Ⅰ,C-ⅢmRNA的表达最明显(P<0.01)。结论:桂枝茯苓丸混悬液可有效减少肝组织α-SMA,TGF-β1,CTGF,C-Ⅰ,C-Ⅲ蛋白和mRNA表达量,有良好的抗肝纤维化作用。
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| 关 键 词： | 肝纤维化 桂枝茯苓丸 |
| 收稿时间： | 2011-04-06 |
Study on Inhibition and Mechanism of Guizhi Fuling Wan on Hepatic Fibrosis Rats |
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| LI Ji,YE Jun,XUE Dong-ying,ZHANG Jie,CHEN Bei,SUN Lin,LIU Zhi-yong and LI Xiao-hong. Study on Inhibition and Mechanism of Guizhi Fuling Wan on Hepatic Fibrosis Rats[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(24): 171-175 |
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| Authors: | LI Ji YE Jun XUE Dong-ying ZHANG Jie CHEN Bei SUN Lin LIU Zhi-yong LI Xiao-hong |
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| Affiliation: | Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China;Department of Infectious Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medical, Shanghai 200062, China |
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| Abstract: | |
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| Keywords: | hepatic fibrosis Guizhi Fuling Wan function mechanism |
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