复方浙贝颗粒联合阿霉素对K562／A02移植瘤mdr1基因表达的影响

目的：探讨复方浙贝颗粒联合阿霉素对K562／A02移植瘤多药耐药基因mdr1表达的影响。方法：将人红白血病多药耐药细胞系K562／A02细胞接种于BALB／c—nu裸小鼠腋前皮下构建多药耐药移植瘤模型，给予不同剂量复方浙贝颗粒灌胃联合阿霉素腹腔注射治疗。治疗14d后处死小鼠，剥离肿瘤，检测各组移植瘤的瘤质量，计算抑瘤率；荧光定量聚合酶链式反应法检测各组移植瘤mdr1基因表达，2^-△△Ct法计算各组mdr1倍增变化率。结果：各剂量复方浙贝颗粒联合阿霉素组的瘤质量明显低于模型组（P〈0．05）；高、中剂量复方浙贝颗粒联合阿霉素组的瘤质量明显低于阿霉素组（P〈0．05）；高、中剂量复方浙贝颗粒联合阿霉素组移植瘤mdr1基因表达较阿霉素组明显减少（P〈0．05）。结论：复方浙贝颗粒（高、中剂量）联合阿霉素能够减少多药耐药移植瘤mdr1基因的表达。

Effects of Compound Zhebei Granule plus doxorubicin on mdr1 gene expression in nude mice with K562/A02 tumor xenografts

Objective： To investigate the effects of Compound Zhebei Granule （CZBG）, a compound traditional Chinese herbal medicine, combined with adriamycin （ADM） on mdr1 gene expression in tumor xenografts in nude mice.
Methods： A tumor xenografts model was established by subcutaneously injecting multidrug resistance cell line K562/A02 into the axillary flank of BALB/c-nu mice. Sixty tumor-bearing nude mice were divided into untreated group, ADM group and high-, medium-, and low-dose CZBG plus ADM groups. The nude mice in the CZBG plus ADM groups were intragastrically administered with CZBG once per day and intraperitoneally injected with ADM once every other day. The nude mice in the untreated group and the ADM group were administered with normal saline or ADM respectively. The course of treatment was continuous for 14 days. The weight of tumor xenografts was measured after treatment and the inhibition rate of tumor xenografts was calculated. The mdr1 gene expression in tumor xenografts was tested by quantitative polymerase chain reaction method.
Results： Compared with the untreated group, CZBG （high-, medium- and low-dose） plus ADM could significantly decrease the weight of tumor xenografts in nude mice （P〈0.05）. Compared with ADM, high- and medium-dose CZBG plus ADM could decrease the weight of tumor xenografts in nude mice with statistical significance （P〈0.05）. The high- and medium-dose CZBG plus ADM could also significantly decrease the mdrl gene expression in tumor xenografts as compared with ADM alone （P〈0.05）.
Conclusion： CZBG at high- and medium-dose plus ADM can inhibit the growth of tumor xenografts and decrease the mdr1 gene expression in tumor xenoarafts in nude mice.