三七芪连汤治疗萎缩性胃炎有效部位纯化工艺研究

目的:考察5种大孔吸附树脂对三七芪连汤(三七、黄芪、黄连)治疗萎缩性胃炎有效部位--总皂苷和总生物碱的吸附分离性能,确定大孔吸附树脂吸附分离三七芪连汤总皂苷和总生物碱的工艺条件.方法:以总皂苷和总生物碱吸附量、含量和回收率为考察指标;总皂苷和总生物碱含量测定、泄漏曲线和洗脱曲线考察均采用紫外-可见分光光度法.结果:AB-8型大孔吸附树脂对三七芪连汤总皂苷及总生物碱有良好吸附分离性能,适宜的工艺条件为:复方提取物上样浓度为40mg·mL-1,总皂苷和总生物碱最大吸附量(单位树脂湿体积吸附量)分别为5.28 mg·mL-1和12.38 mg·mL-1,洗脱剂为50%乙醇,洗脱剂用量为8倍量树脂体积,洗脱流速为2 mL·min-1,树脂经95%乙醇和1 mol·L-1NaOH再生后,可重复使用4次.结论:AB-8型大孔吸附树脂在所确定的工艺条件下,可较好地吸附分离三七芪连汤总皂苷和总生物碱,乙醇洗脱物中总皂苷含量达20%以上,总生物碱含量达30%以上,总皂苷和总生物碱的回收率分别达75%和65%以上.

Effect of purification of the effective fractions of Sanqi Qilian Decoction on atrophic gastritis

AIM: To investigate the adsorptive and separative properties of 5 kinds of macroporous adsorbing resins to the total saponins and alkaloids, which treated atrophic gastritis with Sanqi Qilian Decoction (Radix et Rhizoma Notoginseng, Radix Astragali, Rhizoma Coptidis.) METHODS: The adsorption capacities, contents and recoveries of the total saponins and alkaloids were selected as the evaluating quotas. The determination of the contents of the total saponins and alkaloids and the investigation into leakage and elution curve were completed with UV-VIS spectrophotometric method. RESULTS: AB-8 resin had the best adsorptive and separative properties to the total saponins and alkaloids in Sanqi Qilian Decoction. The applicable technology was as follows: the concentration of original solution of the extract of Sanqi Qilian Decoction was 40 mg/mL; the largest adsorption capacities on resin to the total saponins and alkaloids were 5.28 mg/mL and 12.38 mg/mL respectively; the eluant was 50% ethanol with 8 times of resin volume, and the elution velocity was 2 mL/min. AB-8 resin could be used for 4 times repeatedly after being reproducted by 95% ethanol and 1 mol/L NaOH. CONCLUSION: Under the process condition which had been selected above, AB-8 resin can be used successfully to adsorb and separate with over 20% of the content of the total saponins Sanqi Qilian Decoction, and 30% of the content of the total alkaloids in the 50% ethanol eluate, and with over 75% of total saponins recovery and 65% of total alkaloids recovery respectively.