脑卒中患者血浆HDL亚类组成及与血压的相关性

目的研究脑卒中患者高密度脂蛋白(high density lipoprotein,HDL)亚类组成及相对百分含量的变化,并探讨脑卒中患者血压与HDL亚类组成的关系。方法采用双向电泳-免疫印迹检测法分析了79例正常对照和98例脑卒中患者HDL亚类组成及相对百分含量。结果脑梗死组preβ1-HDL(P<0.05或P<0.001)、HDL3c(P<0.001)水平显著增加,而HDL2a(P<0.05)及HDL2b(P<0.05或P<0.001)水平显著减少。脑出血组preβ1-HDL、HDL3c含量显著增加(P<0.05),而HDL2b含量显著减少(P<0.001)。受试者按收缩压水平分组发现,随着收缩压水平逐渐升高,小颗粒的preβ1-HDL、HDL3c及HDL3a水平均有升高趋势,而大颗粒的HDL2b水平有降低趋势。与正常组比较,收缩压高组(组Ⅳ:160 mmHg≤收缩压<180 mmHg)及收缩压极高组(组V:收缩压≥180 mmHg)preβ1-HDL、HDL3c含量均显著升高(P<0.05),而HDL2b含量显著降低(P<0.05)。且收缩压水平与preβ1-HDL、HDL3c水平呈正相关(r=0.235,r=0.250),而与HDL2b水平呈负相关(r=-0.228)。结论脑卒中患者HDL颗粒直径呈变小趋势,并且随着收缩压水平升高,其HDL颗粒的变小程度更加明显。

Study on the Distribution of Plasma HDL Subclasses and the Relationship with the Blood Pressure in Stroke Patients

AimTo study the distribution of plasma HDL subclasses in stroke patients and the relationship between the levels of the blood pressure and the contents of plasma HDL subclasses in stroke patients.MethodsThe contents of HDL subclasses in 79 healthy controls and 98 stroke subjects were determined by two-dimensional gel electrophoresis associated with immunodetection method.ResultsIn contrast to healthy controls,the contents of preβ1-HDL and HDL3c increased significantly and that of HDL2a and HDL2b were decreased significantly in the cerebral infarction patients(P<0.05).The levels of preβ1-HDL and HDL3c were significantly higher and HDL2b level was significantly lower(P<0.001) in cerebral hemorrhage subjects.With the levels of systolic pressure increaing,the contents of preβ1-HDL,HDL3c and HDL3a increased and that of HDL2b decreased.When the pressure was greater than 160 mmHg,the contents of preβ1-HDL and HDL3c were significantly higher than healthy controls(P<0.05),and the contents of HDL2b were significantly lower than healthy controls(P<0.05).Systolic pressure was positively correlated with preβ1-HDL and HDL3c(r=0.235 and 0.250 respectively) and negatively correlated with HDL2b(r=-0.228 respectively).ConclusionsThe particle size of HDL in stroke subjects showed a shift toward smaller size in stroke subjects,and the tendency was related with the increase of systolic pressure levels.