HPLC法测定附子不同炮制品、川乌、参附注射液中腺苷的含量

目的:首次从附子中分得腺苷,并应用高效液相色谱法建立附子不同炮制品、川乌、参附注射液中腺苷含量的测定方法。方法:采用Diamonsil Cl8柱(250 mm×4.6 mm,5μm);流动相:甲醇-磷酸盐缓冲液(15:85);检测波长:260 nm;流速:1.0 mL/min。结果:腺苷在0.062~0.217μg范围内线性关系良好,R=0.9997,平均回收率为99.9%,RSD=2.41%。结论:本方法可靠、准确、简便,为评价附子不同炮制品、川乌、参附注射液的质量提供了科学依据。

Determination of Adenosine in different Aconiti Lateralis Radix Praeparata,Aconiti Radix and Shenfu Injectionby HPLC

Objective： The study was aimed to establish a HPLC method for the determination of Adenosine which was isolated from the Aconiti Lateralis Radix for the first time in different processed products of Aconiti Lateralis Radix Praeparata, Aconiti Radix and Shenfu Injection. Method： The separation of the determination was achieve on a Diamonsil C18 column （250 mm×4.6 mm, 5 μm）. The mobile phase was MeOH-phosphate buffer （15：85） at a flow-rate of 1.0 mL·min^-1. The detector was set at UV 260 nm. Result： The method had a good linear relationship in the range from 0.062 μg to 0.217μg （R=0.9997）. The average recovery was 99.9% and RSD was 2.41%. Conclusion： The method is reliable, stable, simple and can be used to evaluate the quality of different processed products of Aconiti Lateralis Radix Praeparata, Aconiti Radix and Shenfu Injection.