CD31标记的微血管密度在乳腺浸润性导管癌中的表达及其临床意义

目的：探讨CD31标记的微血管密度（ MVD-CD31）在乳腺浸润性导管癌组织中表达的临床意义。方法应用SP免疫组织化学法分别检测2012年5~10月河北医科大学第四医院乳腺中心诊治的乳腺浸润性导管癌120例及乳腺纤维腺瘤30例组织标本中的MVD-CD31的表达,并应用t检验和方差分析研究乳腺浸润性导管癌组织中MVD-CD31与临床生物学特征和Ki67的关系。结果乳腺浸润性导管癌组织中的 MVD-CD31高于纤维腺瘤组织（16．586＆#177;9．528比10．403＆#177;3．052,t=3．724, P=0．005）。乳腺浸润性导管癌组织中MVD-CD31的表达与肿瘤直径、TNM分期有关（ t=3．761,P=0．000；F=2．983, P=0．032）,与月经、有无淋巴结转移、有无脉管瘤栓、ER、PR、HER-2表达及组织学分级无关（t=0．754、-0．533、1．633、1．853、1．040、0．276, F=1．937； 〉0．05）。三阴性乳腺浸润性导管癌组中MVD-CD31表达明显高于非三阴性组（t=2．078,P=0．043）。乳腺浸润性导管癌患者Ki67＆gt;14%组的MVD-CD31表达显著高于Ki67≤14%组（t=-2．287, P=0．030）, MVD-CD31和Ki67表达呈正相关（r=0．356, P=0．011）。结论 MVD-CD31可以反映乳腺浸润性导管癌的生物学行为,其检测结果对乳腺癌患者的病情评估、判断预后具有重要意义。

Density of microvessels marked by CD31 in breast invasive ductal carcinoma and its clinical significance

Objective To investigate the density of microvessels indicated by CD31 （ MVD-CD31 ） in breast invasive ductal carcinoma and explore its clinical significance. Methods We detected MVD-CD31 by SP immunohistochemical method in tissue samples from 120 cases of breast invasive ductal carcinoma and 30 cases of breast fibroadenoma in Breast Disease Center, the Fourth Affiliated Hospital, Hebei Medical University from May to October in 2012. t test and variance analysis were used to analyze the correlation of MVD-CD31 with clinical biological characteristics and Ki67 level. Results MVD-CD31 in breast invasive ductal carcinoma was significantly higher than that in breast fibroadenoma（16. 586＆#177;9. 528 vs 10. 403＆#177;3. 052, t=3. 724, P=0. 005）. In breast invasive ductal carcinoma, MVD-CD31 was correlated with tumor diameter and TNM staging （ t=3. 761,P=0. 000;F=2. 983, P=0. 032）, but not correlated with menstruation, lymph node metastasis, vascular invasion, ER, PR and HER-2 expression and histological grade （ t=0. 754,-0. 533,1. 633,1. 853, 1. 040,0. 276, F=1. 937; 〉0. 05）. In triple negative breast invasive ductal carcinoma, MVD-CD31 was significantly higher than that in non-triple-negative group （t=2. 078,P=0. 043）. MVD-CD31 in the patients with Ki67＆gt;14% was significantly higher than that in the patients with Ki67≤14% （t=2. 078, P=0. 043）;There was a positive correlation between MVD-CD31 and Ki67 expression in breast invasive ductal carcinoma （r=0. 356, P=0. 011）. Conclusion MVD-CD31 can reflect the biological behaviors of breast invasive ductal carcinoma, which is helpful to assess general condition and predict the prognosis of breast cancer patients.