黄芪多糖抑制HepG2细胞增殖及可能机制研究

目的 研究黄芪多糖对HepG2细胞增殖的作用及可能机制。 方法 用不同浓度(100、200和400 μg/ml)的黄芪多糖(astragalus polysaccharide,APS)处理HepG2细胞,采用MTT法检测细胞增殖,采用Western blot检测细胞内糖原合成酶激酶3β(glycogen synthase kinase 3β,GSK3β)的表达。 结果 APS100组、APS200组及APS400组HepG2细胞第1~7 d吸光度和GSK3β蛋白质表达均显著低于对照组(P<0.05);APS100组和APS400组HepG2细胞D1~D7吸光度和GSK3β蛋白质表达差异无统计学意义(P>0.05),但均显著高于APS200组(P<0.05)。 结论 黄芪多糖可显著抑制HepG2细胞增殖,其机制可能与抑制糖原合成酶激酶3β表达有关,其中200 μg/ml抑制作用最强。

Inhibitory effect of astragalus polysaccharide on the proliferation of HepG2 cells and its potential mechanism

Objective To study the inhibitory effect of astragalus polysaccharide (APS) on the proliferation of HepG2 cells and its potential mechanism. Methods HepG2 cells were cultured and treated with 100 μg/ml, 200 μg/ml or 400 μg/ml APS. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell proliferation. Western blot was employed to examine the expression of glycogen synthase kinase 3β(GSK3β) . Results The absorbance at the 1^st-7^th day (D1-D7) in MTT assay and GSK3β expression in HepG2 cells at the 5^th hour aftertreatment were significantly lower in the groups of APS100, APS200 and APS400 than in the control group(P<0.05). No statistically significant differences were found in the D1-D7 absorbance and GSK3β expression between the groups of APS100 and APS400 (P>0.05), but both of them were higher than those of the APS200 group (P<0.05). Conclusions Astragalus polysaccharide can significantly inhibit the proliferation of HepG2 cells, and its mechanism may be related to inhibiting GSK3β expression. The results suggest that 200 μg/ml ASP has the best inhibitory effect.