多种粘附分子基因在原发性肝癌的表达及其临床意义

目的:探讨多种粘附分子基因在原发性肝癌中的表达及其临床意义。方法:取64例用RT-PCR法半定量检测肝癌粘附分子的基因表达情况,分析其临床意义。结果:(1)原发性肝癌各粘附分子的表达率分别为:E-钙粘蛋白 90.62%、细胞间粘附分子-1 93.75%、粘附分子CD44 50.00%、粘附分子CD_44V 96.88%、整合素α₅ 100%、整合素β₁ 100%,CD44与其他粘附分子表达率的差异显著。(2)肝癌组织各粘附分子E-钙粘蛋白 、细胞间粘附分子-1 、粘附分子CD44、粘附分子CD_44V 、整合素α₅、整合素β₁ mRNA的表达量分别为:1.24±0.54、0.96±0.37、0.62±0.73、0.86±0.33、 0.97±0.49、1.41±0.24,其中粘附分子CD44与E-钙粘蛋白、整合素β₁的表达量差异显著。(3)肝癌分期与粘附分子基因mRNA表达量的关系显示:E-钙粘蛋白、粘附分子CD44的表达量随分期增高而下降,其中粘附分子CD44Ⅰ-Ⅱ期和Ⅳ期的表达量差别显著;细胞间粘附分子-1、粘附分子CD_44V,整合素α₅、整合素β₁的表达量随分期增高而增高,其中细胞间粘附分子-1Ⅰ-Ⅱ期和Ⅳ期的表达量差别显著。(4)肝癌粘附分子基因mRNA表达量与临床病理生理特点相关分析显示:肝癌粘附分子基因mRNA表达量与肿瘤大小、有无转移、有无包膜和结节数目有关,与AFP水平、肝硬化情况、肝功能状况无关。结论:肝癌粘附分子基因mRNA的表达存在明显的差异,粘附分子CD44表达缺失率高。肝癌粘附分子基因mRNA的表达与肿瘤大小、有无转移、结节数目、有无包膜相关。

Expression of adhesion molecules in hepatocellurlar carcinoma and its clinical significance

AIM: To investigate the expression of adhesion molecules in hepatocellular carcinoma (HCC), and analyze its clinical significance. METHODS: The expressions of adhesion molecules of tumor tissues of 64 cases and adjacent tissues of 12 cases of HCC were detected with RT-PCR. RESULTS: ①The expression rates of E-cadherin, ICAM-1, CD44, CD44V, α₅, β₁ were 90.62%, 93.75%, 50.00%, 96.88%, 100%, 100%, respectively, and there was a significant difference between CD44 and other adhesion molecules. ②The expression level of E-cadherin, ICAM-1, CD44, CD_44V, α₅,β₁ in liver cancer tissues were 1.24±0.54, 0.96±0.37, 0.62±0.73, 0.86±0.33, 0.97±0.49, 1.41±0.24, respectively, and there was a significant difference between CD44 and E-cadherin, β₁. ③The expression level of E-cadherin and CD44 mRNA declined as HCC stage become higher, and there was a statistical difference in the expression level of CD44 mRNA between Ⅰ-Ⅱ stage and Ⅳ stage. The expression level of ICAM-1, α₅, β₁ had a trend to rise as HCC stage become higher, and there was a statistical difference in the expression level of ICAM-1 between Ⅰ-Ⅱ stage and Ⅳ stage. ④The expression level of ICAM-1,CD_44V, α₅, β₁ had positive correlation with tumor volume, tumor nodules, tumor metastasis, and had negative correlation with tumor encapsulation. E-cadherin and CD44 had negative correlation with tumor volume, tumor nodules, tumor metastasis, and had positive correlation with tumor encapsulation. All showed no significant correlation with the level of AFP , the degree of cirrhosis and the function of liver. CONCLUSION: There was a significant difference in the expression level of adhesion molecule mRNA in HCC, and their expression had Spearman correlation with each other. The expression level of adhesion molecule mRNA is associated with tumor volume, tumor nodules and tumor metastasis.