高原缺氧腹泻大鼠血清内毒素变化及其肝功能改变的相关性研究

目的:探讨模拟急进高原缺氧环境对大鼠血清内毒素(endotoxin)水平的影响及与肝功能损害的相关性。方法:将30只雄性Wistar大鼠均分为高原缺氧组和高原腹泻组,每组15只。高原组在平原饲养1个月后于24h内急运至海拔4767米高原,建立高原缺氧模型分别于到达高原后7d取门静脉血和腹主动脉血,分别检测各组间血LPS和肝功能(ALT、AST、TBil)。结果:与高原缺氧组比较,高原腹泻组血中LPS和ALT、AST、TBil均有明显升高(P均<0.01);高原腹泻组LPS升高与ALT、AST、TBil水平呈显著正相关(P<0.05)。结论:高原缺氧环境中,腹泻可加重肠黏膜屏障破坏,可导致肠源性内毒素血症,吸收入血的内毒素可能参与高原缺氧环境肝功能损害过程。

Changes of Serum Endotoxin and Liver Functions of Rats with Diarrhea in High Altitude Hypoxia

Objective:To investigate the effects of simulated acute high altitude hypoxia on the level of rat plasma lipopolysaccharides(LPS) and liver functions.Methods:30 male Wistar rats were equally divided into high altitude hypoxia group and high altitude diarrhea group.The rats of the above two groups were moved to an altitude of 4767 meters above within 24h after being fed on the plain for 1 month,and the model of high altitude hypoxia was established.The portal vein blood of all rats was taken on day 7 after arrival,and their blood LPS,ALT,AST,and TBil were detected.Results:When compared with the high altitude hypoxia group,the levels of LPS,ALT,AST and TBil were significantly higher(all P<0.01) and the level of LPS was positively correlated with the level of ALT,AST,or TBil(P<0.05) in high altitude diarrhea group.Conclusion:In high altitude hypoxia environment,diarrhea may increase the damage of intestinal mucosal barrier and lead to intestinal endotoxemia,and the endotoxin absorbed into the bloodstream may be involved in the process of liver damage.