GLP-2对脓毒症大鼠肠道细菌移位的影响

目的探讨胰高血糖素样肽-2(glucagon like peptide-2,GLP-2)对脓毒症大鼠肠道细菌移位的影响。方法Sprague Dawley大鼠60只,随机分为3组,A组:对照组,B组:内毒素组,C组:治疗组。分别以生理盐水、EcolliO55:B5、GLP-2腹腔注射,留取末端回肠组织检测肠黏膜上皮细胞间紧密连接occludin蛋白表达,肠系膜淋巴结、肝、肺组织匀浆后细菌培养计算细菌移位率,同时观察肠黏膜超微结构变化。结果①电镜下A组肠黏膜微绒毛及紧密连接未见异常,B组紧密连接增宽,微绒毛变细、稀疏,部分断裂、脱落。C组改变较B组明显减轻。②免疫组化平均光密度值及western blot净光密度值结果可见:occludin表达B组明显低于A组,C组明显高于B组。③肠系膜淋巴结、肝、肺器官细菌移位率水平B组明显高于A组,C组明显低于B组。结论 GLP-2可以降低肠道屏障功能损伤的程度,减少细菌移位率,对脓毒症的肠道损伤有一定治疗作用。

Effects of Glucagon-Like Peptide 2 on Intestinal Bacteria Translocation in Rats with Sepsis

Objective To investigate the effects of glucagon like peptide-2 on intestinal bacteria translocation in rats with sepsis.Methods 60 healthy male Sprague-Dawley rats,were randomly divided into three experimental groups: normal control group(group A) were injected with normal saline intraperitoneally,LPS group(group B) and LPS+GLP-2 group(group C) were intraperitoneally treated with LPS and LPS plus GLP-2 respectively.All rats were sacrificed at 6 h after injection,terminal ileum of each rat was collected for the observation of the pathologic changes of intestinal mucosa under transmission electron microscope.The expression of occludin was analyzed by immunohistochemistry and western blot.Homogenated tissues of mesenteric lymph nodes,liver and lung were cultured to determine bacterial tranlocation.Results ①Microvilli and tight junctions were intact in group A.Enlargement of tight junctions were observed in group B and microvilli were thin,rare and shed.The rough endoplastic reticulum,mitochondria,and glycogen particles were damaged.The above changes were alleviated in group C.②The content of occludin was obviously lower in group B than that in group A(P<0.01).While the content of occludin was significantly increased in group C in comparison with group B(P<0.01).③In comparison with group A,bacterial translocation rate in mesenteric lymph nodes,liver,lung in group B was significantly increased(P<0.01).As compared with group B,bacterial translocation rate was makedly decreased in group C(P<0.01).Conclusion Glucagon like peptide-2 can reduce the degree of intestinal mucosa damage and bacterial translocation with sepsis,it played an important role in protecting intestinal mucosa barrier.