无镁诱导培养大鼠海马神经元与SH-SY5Y细胞自发性放电的变化

  目的 利用无镁细胞外液诱导原代培养大鼠海马神经元和SH-SY5Y细胞建立癫痫放电模型。方法 采用新生24 h内Wistar大鼠，分离海马神经元进行原代培养。对于体外培养至10 d的神经元和传代培养的SH-SY5Y细胞，无镁细胞外液处理细胞3 h并恢复正常细胞外液，应用全细胞膜片钳技术记录2种细胞的放电情况。结果 无镁处理后的神经元存在自发性的“癫痫样”放电；SH-SY5Y细胞未出现“癫痫样”放电。结论 细胞间通过突触联系形成网状结构可能是诱导“癫痫样”放电的必要条件之一。

The Spontaneous Discharge of Cultured Rat Hippocampal Neurons and SH-SY5Y Cells Induced by Mg2+-free Extracellular Fluid

Objective To detect the spontaneous discharge of cultured rat hippocampal neurons and SH-SY5Y cells in Mg2+-free extracellular fluid.Methods The postnatal hippocampal tissues were taken from 1-day-old Wistar rats and were used for primary culture.Neurons after 10-day culture and SH-SY5Y cells were treated with Mg2+-free extracellular fluid for 3 h,then the discharge activity was recorded by patch clamp technique.Results Neurons showed spontaneous "epileptiform discharge" after the Mg2+-free extracellular fluid treatment;on the contrary,SH-SY5Y cells did not show spontaneous "epileptiform discharge".Conclusion Synaptic connections between cells through the formation of network structure may be necessary for inducing "epileptiform discharge".