RNA干扰沉默S100A2和E-cadherin基因后对胃癌细胞增殖和侵袭力的影响

 目的研究RNA干扰沉默S100A2和E-cadherin基因后对胃癌细胞增殖和侵袭力的影响。方法采用RNA干扰技术，在E-cadherin和S100A2表达阳性的MNK45细胞同时沉默E-cadherin和S100A2基因表达，逆转录聚合酶链反应和Western blot检测干涉效果，MTT 和Transwell检测细胞生长和侵袭力的变化。结果小干扰RNA明显抑制MKN45 细胞E-cadherin和S100A2 的表达；干涉第11天，E-cadherin和S100A2蛋白表达下降，细胞生长速度和细胞侵袭力明显增加。结论E-cadherin和S100A2蛋白具有抑制细胞增殖和侵袭的功能，可作为抑癌因子参与胃癌的发生与转移。

Effects of Gene Silencing in S100A2 and E-cadherin by RNA Interference on Gastric Cancer Cell Proliferation and Invasiveness

Objective To study the effects of gene silencing in S100A2 and E-cadherin by RNA interference on gastric cancer cell proliferation and invasiveness.Methods The expression of E-cadherin and S100A2 genes was silenced by RNA interference in the cell line MKN-45 in which E-cadherin and S100A2 genes are positively expressed.The effect of RNA interference was detected by RT-PCR and Western blot,and the changes of cancer cell proliferation and invasiveness were detected by MTT and Transwell.The effects of E-cadherin and S100A2 genes on gastric cancer were finally analyzed by RNA interference in the study.Results The results showed that the synthesis of E-cadherin and S100A2 mRNA was suppressed dramatically after 7 days triggered by small interfering RNA.On the 11th day of interference,the expression of E-cadherin and S100A2 protein was lower.MTT assay was performed to observe the cell growth rate.Conclusion E-cadherin and S100A2 work as cancer suppressor genes in the tumorogenesis and metastasis of gastric cancer with the function of inhibiting the proliferation and invasiveness of gastric cancer cell.